Microfluidic devices and methods of preparing and using the same
First Claim
Patent Images
1. A one-step microfluidic device, comprising:
- a photoresist layer defining an inlet chamber adapted to receive a sample fluid to be tested, a reaction chamber in fluid communication with said inlet chamber, at least one detection chamber in fluid communication with said reaction chamber, and an absorbent chamber downstream of said at least one detection chamber in the direction of flow of the sample fluid;
a support structure arranged under said photoresist layer for providing rigid support for said photoresist layer; and
a cover arranged above said photoresist layer for covering said reaction chamber, said at least one detection chamber and said absorbent chamber,wherein said absorbent chamber comprises at least one absorbent channel and said absorbent channel includes an open end; and
wherein the microfluidic device is capable of precise sampling.
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Abstract
Microfluidic devices include a photoresist layer in which an inlet chamber, an optional reaction chamber and at least one detection chamber are in fluid contact, a support arranged under the photoresist layer and a cover arranged above the photoresist layer. The devices further include a set of absorbent channels downstream of the last detection chamber. Biogenic or immunoreactive substances are placed in the reaction chamber and detection chamber(s). When a liquid sample is dropped into the inlet chamber, the sample liquid is drawn through the devices by capillary action. Detection methods include electrochemical detection, colorimetric detection and fluorescence detection.
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Citations
36 Claims
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1. A one-step microfluidic device, comprising:
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a photoresist layer defining an inlet chamber adapted to receive a sample fluid to be tested, a reaction chamber in fluid communication with said inlet chamber, at least one detection chamber in fluid communication with said reaction chamber, and an absorbent chamber downstream of said at least one detection chamber in the direction of flow of the sample fluid; a support structure arranged under said photoresist layer for providing rigid support for said photoresist layer; and a cover arranged above said photoresist layer for covering said reaction chamber, said at least one detection chamber and said absorbent chamber, wherein said absorbent chamber comprises at least one absorbent channel and said absorbent channel includes an open end; and wherein the microfluidic device is capable of precise sampling. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 23, 24, 25, 26, 27, 28, 31, 32)
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19. A one-step microfluidic device, comprising:
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a photoresist layer defining an inlet chamber adapted to receive a sample fluid to be tested, a reaction chamber in fluid communication with said inlet chamber, a mixing channel in fluid communication with said reaction chamber, at least one detection chamber in fluid communication with said reaction chamber, and a set of absorbent channels downstream of said at least one detection chamber in the direction of flow of the sample fluid; a support structure arranged under said photoresist layer for providing rigid support for said photoresist layer; and a cover arranged above said photoresist layer for covering said reaction chamber, said at least one detection chamber and said absorbent channels, wherein said absorbent channels include open ends; and wherein the microfluidic device is capable of precise sampling. - View Dependent Claims (20)
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21. A one-step microfluidic device, comprising:
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a photoresist layer defining an inlet chamber adapted to receive a sample fluid to be tested, a reaction chamber in fluid, communication with said inlet chamber, a mixing channel in fluid communication with said reaction chamber, at least one detection chamber in fluid communication with said reaction chamber, and a set of absorbent channels downstream of said at least one detection chamber in the direction of flow of the sample fluid, wherein said at least one detection chamber further comprises a conductive surface in or defining at least part of said at least one detection chamber; a support structure arranged under said photoresist layer for providing rigid support for said photoresist layer; and a cover arranged above said photoresist layer for covering said reaction chamber, said at least one detection chamber and said absorbent channels, wherein said absorbent channels include open ends; and wherein the microfluidic device is capable of precise sampling. - View Dependent Claims (22)
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29. A one-step electrochemical sensor device, comprising:
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a photoresist layer defining an inlet chamber adapted to receive a sample fluid to be tested, a reaction chamber in fluid communication with said inlet chamber, at least one detection chamber in fluid communication with said inlet chamber, and an absorbent chamber downstream of said at least one detection chamber in the direction of flow of the sample fluid; a support structure arranged under said photoresist layer for providing rigid support for said photoresist layer; a cover arranged above said photoresist layer for covering said reaction chamber, said at least one detection chamber and said absorbent chamber; and a conductive surface in or defining at least part of said at least one detection chamber, wherein said absorbent chamber comprises at least one absorbent channel and said absorbent channel includes an open end; and wherein the microfluidic device is capable of precise sampling. - View Dependent Claims (30)
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33. A one-step microfluidic device, comprising:
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a photoresist layer defining an inlet chamber adapted to receive a sample fluid to be tested, a reaction chamber in fluid communication with said inlet chamber, at least one detection chamber in fluid communication with said reaction chamber, and a set of absorbent channels downstream of said at least one detection chamber in the direction of flow of the sample fluid; a support structure arranged under said photoresist layer for providing rigid support for said photoresist layer; and a cover arranged above said photoresist layer for covering said reaction chamber, said at least one detection chamber and said set of absorbent channels, wherein one or more first biogenic or immunoreactive substances are arranged in said reaction chamber and one or more second biogenic or immunoreactive substances are arranged in each of said at least one detection chamber; wherein said set of absorbent channels defines a plurality of parallel channels communicating at an inlet end with a last one of said at least one detection chamber; wherein said absorbent channels include open ends; and wherein the microfluidic device is capable of precise sampling. - View Dependent Claims (34, 36)
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35. (canceled)
Specification