MICROARRAY SUBSTRATE, METHOD OF USE, AND PRODUCTS COMPRISING THE MICROARRAY SUBSTRATE

• US 20100261621A1
• Filed: 06/28/2010
• Published: 10/14/2010
• Est. Priority Date: 01/03/2006
• Status: Active Grant

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: YOO, Chang-eun, PEAK, Sang-hyun, PARK, Jong-myeon
• Granted Patent: US 8,263,532 B2
• Time in Patent Office: Days
• US Class Current: 506/30
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00722 Nucleotides

Abstract

Provided are a microarray substrate comprising a solid substrate coated with a chemical having a functional residue represented by Formula 1 or 2 below, a method of analyzing a biomolecule using the microarray substrate, and a lab-on-a-chip comprising the microarray substrate:

• • wherein n, the structure within brackets [ ], R₁, R₂, R₃, R₁₀, n and l are as defined in the specification.

Claims

1. A method for analyzing a target biomolecule, comprising:

immobilizing a probe biomolecule to a microarray substrate at a first pH; and

hybridizing a target biomolecule to the immobilized probe biomolecule at a second pH,

wherein the microarray substrate comprises a solid substrate having covalently immobilized thereon a functional residue represented by Formula 1;

2. The method of claim 1, wherein the functional residue is represented by Formula 3 below:

3. The method of claim 2, wherein n is 2.

4. The method of claim 1, wherein the first pH is about 2 to about 3.5, the second pH is about 5 to about 10.

5. The method of claim 1, wherein the solid substrate is made of a material selected from a group consisting of silicon, glass, and a plastic material.

6. The method of claim 1, wherein the biomolecule is a nucleic acid.