CHEMICAL APPROACHES FOR GENERATION OF INDUCED PLURIPOTENT STEM CELLS
First Claim
Patent Images
1. A method of producing an induced pluripotent stem cell from a mammalian non-pluripotent cell, the method comprising contacting the cell with a TGFβ
- receptor/ALK5 inhibitor under conditions to produce the induced pluripotent stem cell from the mammalian non-pluripotent cell.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention provides for identification and use of small molecules to induce pluripotency in mammalian cells as well as other methods of inducing pluripotency.
-
Citations
20 Claims
-
1. A method of producing an induced pluripotent stem cell from a mammalian non-pluripotent cell, the method comprising contacting the cell with a TGFβ
- receptor/ALK5 inhibitor under conditions to produce the induced pluripotent stem cell from the mammalian non-pluripotent cell.
- View Dependent Claims (2, 3, 4, 5, 6, 7)
- 8. A method of producing an induced pluripotent stem cell from a mammalian non-pluripotent cell, the method comprising, contacting the cell with a GSK-3 inhibitor under conditions to produce the induced pluripotent stem cell from the mammalian non-pluripotent cell.
-
12. A eukaryotic cell medium comprising a TGFβ
- receptor/ALK5 inhibitor.
- View Dependent Claims (13, 14, 15, 16, 17, 18)
-
19. A kit for inducing pluripotency in non-pluripotent cells, the kit comprising:
a TGFβ
receptor/ALK5 inhibitor; and(i) one or more of an Oct polypeptide, a Klf polypeptide, a Myc polypeptide, and a Sox polypeptide and/or polynucleotide encoding one or more of an Oct polypeptide, a Klf polypeptide, a Myc polypeptide, and a Sox polypeptide;
or(ii) a MEK inhibitor and/or an Erk inhibitor;
or(iii) both (i) and (ii). - View Dependent Claims (20)
Specification