Sequencing Nucleic Acid Polymers with Electron Microscopy
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Abstract
This invention relates to using an electron microscope to sequence by direct inspection of labeled, stretched DNA. This method will have higher accuracy, lower cost, and longer read length than current DNA sequencing methods.
96 Citations
119 Claims
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1-93. -93. (canceled)
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94. A method of determining the sequence of a nucleic acid, the method comprising:
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(a) providing a nucleic acid having a predetermined configuration; (b) imaging the nucleic acid by electron microscopy to obtain electron microscopy (EM) data; and (c) determining the sequence of the labeled nucleic acid from the EM data. - View Dependent Claims (95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111)
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- 112. A substrate comprising a linear nucleic acid on a surface therefore, wherein the linear nucleic acid comprises consistent base-to-base spacing.
Specification