Sequencing Nucleic Acid Polymers with Electron Microscopy
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Abstract
This invention relates to using an electron microscope to sequence by direct inspection of labeled, stretched DNA. This method will have higher accuracy, lower cost, and longer read length than current DNA sequencing methods.
93 Citations
114 Claims
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1-93. -93. (canceled)
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94. A method of determining the sequence of 20 or more consecutive bases in a nucleic acid, the method comprising:
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(a) providing a nucleic acid of 20 or more bases in length; (b) imaging the nucleic acid by electron microscopy to obtain electron microscopy (EM) data; and (c) determining the sequence of the nucleic acid from the EM data. - View Dependent Claims (95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114)
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Specification