Methods for monitoring multiple gene expression

• US 20100267584A1
• Filed: 04/01/2010
• Published: 10/21/2010
• Est. Priority Date: 10/06/2000
• Status: Active Grant

• Current Assignee: Novozymes, Novozymes A/S (Novozymes)
• Original Assignee: Novozymes, Novozymes A/S (Novozymes)
• Inventors: Lapidus, Alla, Sorokine, Alexei, Bolotine, Alexandre, Clausen, Ib Groth, Berka, Randy M.
• Granted Patent: US 8,168,773 B2
• Time in Patent Office: Days
• US Class Current: 506/16
• CPC Class Codes: C12Q 1/6809 Methods for determination or identification of nucleic acids involving differential detection
• CPC Class Codes: C12Q 1/689 for bacteria
• CPC Class Codes: C12Q 2565/102 Multiple non-interacting labels
• CPC Class Codes: C12Q 2565/501 being an array of oligonucleotides
• CPC Class Codes: C12Q 2600/158 Expression markers

Abstract

The present invention relates to methods for monitoring differential expression of a plurality of genes in a first Bacillus cell relative to expression of the same genes in one or more second Bacillus cells using microarrays containing Bacillus genomic sequenced tags. The present invention also relates to computer readable media and computer-based systems. The present invention further relates to substrates containing an array of Bacillus licheniformis or Bacillus clausii GSTs.

Claims

1. A substrate comprising an array of Bacillus GSTs.

2. The substrate of claim 1, wherein the Bacillus GSTs are selected from the group consisting of Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacillus circulans, Bacillus clausii, Bacillus coagulans, Bacillus firmus, Bacillus lautus, Bacillus lentus, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus stearothermophilus, Bacillus subtilis, and Bacillus thuringiensis GSTs.

3. The substrate of claim 1, wherein the Bacillus GSTs are Bacillus licheniformis GSTs.

4. The substrate of claim 3, wherein the Bacillus licheniformis GSTs are selected from the group consisting of SEQ ID NOs. 1-4448, nucleic acid fragments of SEQ ID NOs. 1-4448, and nucleic acid sequences having at least 85% homology to SEQ ID NOs. 1-4448.

5. The substrate of claim 3, wherein the Bacillus licheniformis GSTs are SEQ ID NOs. 1-4448.

6. The substrate of claim 3, wherein the Bacillus licheniformis GSTs are nucleic acid fragments of SEQ ID NOs. 1-4448.

7. The substrate of claim 3, wherein the Bacillus licheniformis GSTs are nucleic acid sequences having at least 85% homology to SEQ ID NOs. 1-4448.

8. The substrate of claim 7, wherein the Bacillus licheniformis GSTs are nucleic acid sequences having at least 90% homology to SEQ ID NOs. 1-4448.

9. The substrate of claim 8, wherein the Bacillus licheniformis GSTs are nucleic acid sequences having at least 95% homology to SEQ ID NOs. 1-4448.

10. The substrate of claim 1, wherein the Bacillus GSTs are Bacillus clausii GSTs.

11. The substrate of claim 10, wherein the Bacillus clausii GSTs are selected from the group consisting of SEQ ID NOs. 4449-8481, nucleic acid fragments of SEQ ID NOs. 4449-8481, and nucleic acid sequences having at least 85% homology to SEQ ID NOs. 4449-8481.

12. The substrate of claim 10, wherein the Bacillus clausii GSTs are SEQ ID NOs. 4449-8481.

13. The substrate of claim 10, wherein the Bacillus clausii GSTs are nucleic acid fragments of SEQ ID NOs. 4449-8481.

14. The substrate of claim 10, wherein the Bacillus clausii GSTs are nucleic acid sequences having at least 85% homology to SEQ ID NOs. 4449-8481.

15. The substrate of claim 14, wherein the Bacillus clausii GSTs are nucleic acid sequences having at least 90% homology to SEQ ID NOs. 4449-8481.

16. The substrate of claim 15, wherein the Bacillus clausii GSTs are nucleic acid sequences having at least 95% homology to SEQ ID NOs. 4449-8481.