EXTRACTION OF SOLUBLES AND MICROBIAL GROWTH STIMULANTS FROM LIGNOCELLULOSIC BIOMASS AND METHODS RELATED THERETO
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Abstract
A method for producing a microbial growth stimulant (MGS) from a plant biomass is described. In one embodiment, an ammonium hydroxide solution is used to extract a solution of proteins and ammonia from the biomass. Some of the proteins and ammonia are separated from the extracted solution to provide the MGS solution. The removed ammonia can be recycled and the proteins are useful as animal feeds. In one embodiment, the method comprises extracting solubles from pretreated lignocellulosic biomass with a cellulase enzyme-producing growth medium (such T. reesei) in the presence of water and an aqueous extract.
76 Citations
43 Claims
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1-9. -9. (canceled)
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10. A method comprising:
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treating plant biomass containing solubles with an ammonia pretreatment to provide a treated plant biomass containing solubles, wherein the plant biomass is from one or more plants; extracting at least a portion of the solubles from plant biomass for use as a microbial growth stimulant. - View Dependent Claims (11, 12, 13, 18, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35)
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14-17. -17. (canceled)
- 19. A method comprising using solubles extracted from pretreated lignocellulosic biomass as a growth medium for an enzyme-producing microorganism, wherein the solubles are extracted using water or aqueous ammonium hydroxide.
Specification