Sequencing Nucleic Acid Polymers with Electron Microscopy
1 Assignment
0 Petitions
Accused Products
Abstract
This invention relates to using an electron microscope to sequence by direct inspection of labeled, stretched DNA. This method will have higher accuracy, lower cost, and longer read length than current DNA sequencing methods.
131 Citations
121 Claims
-
1-93. -93. (canceled)
-
94. A system for determining a sequence of a nucleic acid, the system comprising:
-
(a) a data analysis module configured to; (i) receive electron microscopy (EM) data obtained by imaging a contrast agent labeled nucleic acid with an electron microscope; and (ii) determine a sequence of the nucleic acid from the EM data; and (b) an output configured to provide the sequence of the nucleic acid to a user; wherein the system is configured to provide the sequence at a rate of 1,000 bases per second or greater. - View Dependent Claims (95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114)
-
- 115. An electron microscope imaging substrate comprising a nucleic acid having consistent base-to-base spacing over a length of 1000 base pairs on a surface thereof.
- 118. A needle comprising a tip member having a radius of curvature less than about 200 nm that has been functionalized with a nucleic acid binding compound.
Specification