ASSAY METHOD UTILIZING POLYNUCLEOTIDE SEQUENCES
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Accused Products
Abstract
A method of detecting in a sample an analyte (A) having a molecularly recognizable portion thereon, which comprises: providing (B) a molecular bridging entity having thereon: (i) a portion capable of recognizing the molecularly recognizable portion on the analyte; and (ii) a portion comprising a polynucleotide sequence; and (C) a signalling entity having thereon: (i) a polynucleotide portion capable of annealing to the polynucleotide portion of the bridging entity, thereby to form a stable polynucleotide hybrid, and (ii) a signal generating portion; forming a complex comprising: (1) the analyte (A) complexed through its molecularly recognizable portion to (2) the recognizing portion of the entity (B); the entity (B) being complexed through the polynucleotide portion thereon to (3) the polynucleotide portion of the signalling entity; and detecting a signal by means of the signal generating portion present in the complex.
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Citations
548 Claims
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1-282. -282. (canceled)
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283. A composition of matter comprising:
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a first part which comprises a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
a second part which comprises one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal. - View Dependent Claims (295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 359, 439, 441, 442, 461, 462, 463, 542, 543)
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284. A composition of matter comprising:
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a first part which comprises an analyte having one or more molecularly recognizable portions thereon;
a second part which comprises a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with said molecularly recognizable analyte portion, and a second portion comprising one or more nucleic acid sequences or segments; and
a third part which comprises one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion or portions on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal. - View Dependent Claims (358)
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285. A composition of matter comprising:
a complex which comprises;
a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal.
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286. A composition of matter comprising:
a complex which comprises;
an analyte having one or more molecularly recognizable portions thereon;
a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with said molecularly recognizable analyte portion and a second portion comprising one or more nucleic acid sequences or segments; and
one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal.
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287. A composition of matter comprising:
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a first part which comprises more than one molecular bridging entity, each such entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
a second part which comprises one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said more than one bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal.
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288. A composition of matter comprising:
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a first part which comprises an analyte having one or more molecularly recognizable portions thereon;
a second part which comprises more than one molecular bridging entity, each such entity comprising a first portion capable of recognizing and binding to or hybridizing with said molecularly recognizable analyte portion and a second portion comprising one or more nucleic acid sequences or segments; and
a third part which comprises one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said more than one bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal.
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289. A composition of matter comprising a complex which comprises:
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more than one molecular bridging entity, each such entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said more than one bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal.
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290. A composition of matter comprising a complex which comprises:
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an analyte having one or more molecularly recognizable portions thereon;
more than one molecular bridging entity, each such entity comprising a first portion capable of recognizing and binding to or hybridizing with said molecularly recognizable analyte portion, and a second portion comprising one or more nucleic acid sequences or segments; and
one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said more than one bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal.
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291. A composition of matter comprising:
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a first part which comprises a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
a second part which comprises one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more chemically modified or artificially altered polynucleotides capable of providing a detectable signal. - View Dependent Claims (438)
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292. A composition of matter comprising a complex which comprises:
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a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more chemically modified or artificially altered polynucleotides capable of providing a detectable signal.
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293. A composition of matter comprising:
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a first part which comprises an analyte having one or more molecularly recognizable portions thereon;
a second part which comprises a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
a third part which comprises one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more chemically modified or artificially altered polynucleotides capable of providing a detectable signal.
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294. A composition of matter comprising:
a complex which comprises;
an analyte having one or more molecularly recognizable portions thereon;
a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more chemically modified or artificially altered polynucleotides capable of providing a detectable signal.
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360. An article of manufacture comprising:
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a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
more than one non-radioactive signalling entity, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity second portion nucleic acid sequences or segments, and one or more signal generating portions, each capable of providing a detectable signal. - View Dependent Claims (362)
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361. An article of manufacture comprising:
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a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
more than one non-radioactive signalling entity, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity second portion nucleic acid sequences or segments, and one or more polynucleotides which have been chemically modified or artificially altered.
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363. (canceled)
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366-381. -381. (canceled)
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384-399. -399. (canceled)
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402. (canceled)
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405. (canceled)
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408. (canceled)
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411. A kit for the detection in a sample of an analyte having one or more molecularly recognizable portions thereon, comprising as components thereof:
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(i) a container carrying a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with said molecularly recognizable portion on said analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
(ii) a container carrying more than one non-radioactive signalling entity, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity second portion nucleic acid sequence or segment, and one or more signal generating portions, each such portion being capable of providing a detectable signal. - View Dependent Claims (418, 420, 421, 422, 426, 428, 429, 432, 433, 434, 436, 437, 538, 539, 544, 545)
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412. A kit for the detection in a sample of an analyte having one or more molecularly recognizable portions thereon, comprising as components thereof:
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a container carrying a complex which comprises;
a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with said molecularly recognizable portion, and a second portion comprising one or more nucleic acid sequences or segments; and
more than one non-radioactive signalling entity, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity second portion, and one or more signal generating portions, each such portion being capable of providing a detectable signal.
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413. A kit for the detection in a sample of an analyte having one or more molecularly recognizable portions thereon, comprising as components thereof:
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a container carrying more than one molecular bridging entity, each such entity comprising a first portion capable of recognizing and binding to or hybridizing with molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
a container carrying more than one non-radioactive signalling entity, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion to form a polynucleotide hybrid, and one or more signal generating portions, capable of providing a detectable signal.
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414. A kit for the detection in a sample of an analyte having one or more molecularly recognizable portions thereon comprising as components thereof:
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more than one molecular bridging entity, each such entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
more than one non-radioactive signalling entity, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal.
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415. A kit for the detection in a sample of an analyte having one or more molecularly recognizable portions thereon, comprising as components thereof:
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a complex which comprises;
(i) more than one molecular bridging entity, each such, entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
(ii) more than one non-radioactive signalling entity, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal.
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416. A kit for the detection in a sample of an analyte having one or more molecularly recognizable portions thereon, comprising as components thereof:
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a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
more than one non-radioactive signalling entity, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more polynucleotides which have been chemically modified or artificially altered. - View Dependent Claims (419, 423, 424, 425, 427, 430, 431, 435)
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417. A kit for the detection in a sample of an analyte having one or more molecularly recognizable portions thereon, comprising as components thereof:
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a complex which comprises;
a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
more than one non-radioactive signalling entity, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion to form a polynucleotide hybrid, and one or more polynucleotides which have been chemically modified or artificially altered.
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440. (canceled)
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443. A process for detecting an analyte having one or more molecularly recognizable portions thereon, comprising:
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providing a composition of matter comprising;
a first part which comprises a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
a second part which comprises one or more signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal;
forming a complex comprising said composition and said analyte; and
detecting said analyte by a signal provided by said signal generating portion or portions present in said complex. - View Dependent Claims (364, 365, 488, 532, 533, 534, 535, 536, 537, 540, 541)
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444. A process for detecting an analyte having one or more molecularly recognizable portions thereon, comprising:
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providing a composition of matter comprising a complex which comprises;
a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
one or more signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal;
forming a complex comprising said composition and said analyte; and
detecting said analyte by a signal provided by said signal generating portion or portions present in said complex. - View Dependent Claims (489)
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445. A process for detecting an analyte having one or more molecularly recognizable portions thereon, comprising:
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providing a composition of matter comprising;
a first part which comprises more than one molecular bridging entity, each such entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
a second part which comprises one or more signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said more than one bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal; and
forming a complex comprising said composition and said analyte; and
detecting said analyte by a signal provided by said signal generating, portion or portions present in said complex. - View Dependent Claims (490)
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446. A process for detecting an analyte having one or more molecularly recognizable portions thereon, comprising:
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providing a composition of matter comprising a complex which comprises;
more than one molecular bridging entity, each such entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
one or more signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said more than one bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal;
forming a complex comprising said composition and said analyte; and
detecting said analyte by a signal provided by said signal generating portion or portions present in said complex. - View Dependent Claims (491)
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447. A process for detecting an analyte having one or more molecularly recognizable portions thereon, comprising:
providing a composition of matter comprising;
a first part which comprises a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
a second part which comprises one or more signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more chemically modified or artificially altered polynucleotides capable of providing a detectable signal;
forming a complex comprising said composition and said analyte; and
detecting said analyte by a signal provided by said signal generating portion or portions present in said complex. - View Dependent Claims (492)
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448. A process for detecting an analyte having one or more molecularly recognizable portions thereon, comprising:
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providing a composition of matter comprising a complex which comprises;
a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
one or more signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more chemically modified or artificially altered polynucleotides capable of providing a detectable signal;
forming a complex comprising said composition and said analyte; and
detecting said analyte by a signal provided by said signal generating portion or portions present in said complex. - View Dependent Claims (493)
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449. A process for detecting an analyte having one or more molecularly recognizable portions thereon, comprising:
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providing a composition of matter comprising;
a first part which comprises an analyte having one or more molecularly recognizable portions thereon;
a second part which comprises a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with said molecularly recognizable analyte portion, and a second portion comprising one or more nucleic acid sequences or segments; and
a third part which comprises one or more signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion or portions on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal;
forming a complex comprising the components of said composition and said analyte; and
detecting said analyte by a signal provided by said signal generating portion or portions present in said complex. - View Dependent Claims (382, 383, 494)
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450. A process for detecting an analyte having one or more molecularly recognizable portions thereon, comprising:
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providing a composition of matter comprising a complex which comprises;
an analyte having one or more molecularly recognizable portions thereon;
a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with said molecularly recognizable analyte portion and a second portion comprising one or more nucleic acid sequences or segments; and
one or more signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal;
forming a complex comprising the components of said composition and said analyte; and
detecting said analyte by a signal provided by said signal generating portion or portions present in said complex. - View Dependent Claims (495)
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451. A process for detecting an analyte having one or more molecularly recognizable portions thereon, comprising:
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providing a composition of matter comprising;
a first part which comprises an analyte having one or more molecularly recognizable portions thereon;
a second part which comprises more than one molecular bridging entity, each such entity comprising a first portion capable of recognizing and binding to or hybridizing with said molecularly recognizable analyte portion and a second portion comprising one or more nucleic acid sequences or segments; and
a third part which comprises one or more signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said more than one bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal;
forming a complex comprising the components of said composition and said analyte; and
detecting said analyte by a signal provided by said signal generating portion or portions present in said complex. - View Dependent Claims (496)
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452. A process for detecting an analyte having one or more molecularly recognizable portions thereon, comprising:
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providing a composition of matter comprising a complex which comprises;
an analyte having one or more molecularly recognizable portions thereon;
more than one molecular bridging entity, each such entity comprising a first portion capable of recognizing and binding to or hybridizing with said molecularly recognizable analyte portion and a second portion comprising one or more nucleic acid sequences or segments; and
one or more signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said more than one bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal;
forming a complex comprising the components of said composition and said analyte; and
detecting said analyte by a signal provided by said signal generating portion or portions present in said complex. - View Dependent Claims (497)
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453. A process for detecting an analyte having one or more molecularly recognizable portions thereon, comprising:
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providing a composition of matter comprising;
a first part which comprises an analyte having one or more molecularly recognizable portions thereon;
a second part which comprises a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
a third part which comprises one or more signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more chemically modified or artificially altered polynucleotides capable of providing a detectable signal;
forming a complex comprising the components of said composition and said analyte; and
detecting said analyte by a signal provided by said signal generating portion or portions present in said complex. - View Dependent Claims (498)
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454. A process for detecting an analyte having one or more molecularly recognizable portions thereon, comprising;
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providing a composition of matter comprising a complex which comprises;
an analyte having one or more molecularly recognizable portions thereon;
a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
one or more signalling entitles substantially, incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more chemically modified or artificially altered polynucleotides capable of providing a detectable signal;
forming a complex comprising the components of said composition and said analyte; and
detecting said analyte by a signal provided by said signal generating portion or portions present in said complex. - View Dependent Claims (499)
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455. A process for detecting an analyte having one or more molecularly recognizable portions thereon, comprising:
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providing a composition which comprises;
a first part which comprises a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
a second part which comprises one or more signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal;
fixing or immobilizing said analyte or a sample containing said analyte to a solid support;
forming a complex comprising, said composition and said analyte; and
detecting said analyte by a signal provided by said signal generating portion or portions present in said complex. - View Dependent Claims (400, 401, 403, 404, 500)
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456. A process for detecting an analyte having one or more molecularly recognizable portions thereon, comprising:
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providing a composition of matter comprising a complex which comprises;
a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
one or more signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more signal generating portion capable of providing a detectable signal;
fixing or immobilizing said analyte or a sample containing said analyte to a solid support;
forming a complex comprising said composition and said analyte; and
detecting said analyte by a signal provided by said signal generating portion or portions present in said complex. - View Dependent Claims (501)
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457. A process for detecting an analyte having one or more molecularly recognizable portions thereon, comprising:
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providing a composition comprising;
a first part which comprises more than one molecular bridging entity, each such entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
a second part which comprises one or more signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said more than one bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal;
fixing or immobilizing said analyte or a sample containing said analyte to a solid support;
forming a complex comprising said composition and said analyte; and
detecting said analyte by a signal provided by said signal generating portion or portions present in said complex. - View Dependent Claims (502)
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458. A process for detecting an analyte having one or more molecularly recognizable portions thereon, comprising:
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providing a composition comprising;
more than one molecular bridging entity, each such entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
one or more signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said more than one bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal;
fixing or immobilizing said analyte or a sample containing said analyte to a solid support;
forming a complex comprising said composition and said analyte; and
detecting said analyte by a signal provided by said signal generating portion or portions present in said complex. - View Dependent Claims (503)
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459. A process for detecting an analyte having one or more molecularly recognizable portions thereon, comprising:
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fixing or immobilizing said analyte or a sample containing said analyte to a solid support;
providing a composition comprising;
a first part which comprises a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
a second part which comprises one or more signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more chemically modified or artificially altered polynucleotides capable of providing a detectable signal;
forming a complex comprising said composition and said analyte; and
detecting said analyte by a signal provided by means of said signal generating portion or portions present in said complex. - View Dependent Claims (406, 407, 409, 410, 504)
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460. A process for detecting an analyte having one or more molecularly recognizable portions thereon, comprising:
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fixing or Immobilizing said analyte or a sample containing said analyte to a solid support;
providing a composition comprising a complex which comprises;
a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
one or more signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more chemically modified or artificially altered polynucleotides capable of providing a detectable signal;
forming a complex comprising said composition and said analyte; and
detecting said analyte by a signal provided by means of said signal generating portion or portions present in said complex. - View Dependent Claims (505)
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464. A composition of matter comprising:
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a first part which comprises a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, said analyte molecularly recognizable portion comprising a biological system selected from the group consisting of a virus or a viral component thereof and a cell or a cellular component thereof, said cell or cellular component thereof comprising a bacterium or a bacterial component thereof, said first portion being selected from the group consisting of an antigen, a polyclonal or a monoclonal antibody, a hormone, a receptor, an enzyme, an allosteric effector, an enzyme substrate, an enzyme cofactor, a protein and a protein receptor, and a second portion comprising one or more nucleic acid sequences or segments; and
a second part which comprises one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more signal generating portions capable of directly or indirectly providing a detectable signal, said direct signal providing signal generating portion being selected from the group consisting of a fluorogenic compound, a phosphorescent compound, a chromogenic compound, a chemiluminescent compound, an electron dense compound, an enzyme, and said indirect signal providing signal generating portion being selected from the group consisting of an antibody, an antigen, a hapten, a receptor, a ligand, an enzyme, a polynucleotide sequence capable of recognizing a signal-containing moiety, and a compound capable of binding to an insoluble phase. - View Dependent Claims (476, 477, 478)
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465. A composition of matter comprising:
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a first part which comprises an analyte having one or more molecularly recognizable portions thereon, said analyte molecularly recognizable portion comprising a biological system selected from the group consisting of a virus or a viral component thereof and a cell or a cellular component thereof, said cell or cellular component thereof comprising a bacterium or a bacterial component thereof;
a second part which comprises a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with said molecularly recognizable analyte portion, said first portion being selected from the group consisting of an antigen, a polyclonal or a monoclonal antibody, a hormone, a receptor, an enzyme, an allosteric effector, an enzyme substrate, an enzyme cofactor, a protein and a protein receptor, and a second portion comprising one or more nucleic acid sequences or segments; and
a third part which comprises one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion or portions on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more signal generating portions capable of directly or indirectly providing a detectable signal, said direct signal providing signal generating portion being selected from the group consisting of a fluorogenic compound, a phosphorescent compound, a chromogenic compound, a chemiluminescent compound, an electron dense compound, an enzyme, and said indirect signal providing signal generating portion being selected from the group consisting of an antibody, an antigen, a hapten, a receptor, a ligand, an enzyme, a polynucleotide sequence capable of recognizing a signal-containing moiety, and a compound capable of binding to an insoluble phase.
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466. A composition of matter comprising:
a complex which comprises;
a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, said analyte molecularly recognizable portion comprising a biological system selected from the group consisting of a virus or a viral component thereof and a cell or a cellular component thereof, said cell or cellular component thereof comprising a bacterium or a bacterial component thereof, said first portion being selected from the group consisting of an antigen, a polyclonal or a monoclonal antibody, a hormone, a receptor, an enzyme, an allosteric effector, an enzyme substrate, an enzyme cofactor, a protein and a protein receptor, and a second portion comprising one or more nucleic acid sequences or segments; and
one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more signal generating portions capable of directly or indirectly providing a detectable signal, said direct signal providing signal generating portion being selected from the group consisting of a fluorogenic compound, a phosphorescent compound, a chromogenic compound, a chemiluminescent compound, an electron dense compound, an enzyme, and said indirect signal providing signal generating portion being selected from the group consisting of an antibody, an antigen, a hapten, a receptor, a ligand, an enzyme, a polynucleotide sequence capable of recognizing a signal-containing moiety, and a compound capable of binding to an insoluble phase.
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467. A composition of matter comprising:
a complex which comprises;
an analyte having one or more molecularly recognizable portions thereon;
a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with said molecularly recognizable analyte portion, said analyte molecularly recognizable portion comprising a biological system selected from the group consisting of a virus or a viral component thereof and a cell or a cellular component thereof, said cell or cellular component thereof comprising a bacterium or a bacterial component thereof, said first portion being selected from the group consisting of an antigen, a polyclonal or a monoclonal antibody, a hormone, a receptor, an enzyme, an allosteric effector, an enzyme substrate, an enzyme cofactor, a protein and a protein receptor, and a second portion comprising one or more nucleic acid sequences or segments; and
one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more signal generating portions capable of directly or indirectly providing a detectable signal, said direct signal providing signal generating portion being selected from the group consisting of a fluorogenic compound, a phosphorescent compound, a chromogenic compound, a chemiluminescent compound, an electron dense compound, an enzyme, and said indirect signal providing signal generating portion being selected from the group consisting of an antibody, an antigen, a hapten, a receptor, a ligand, an enzyme, a polynucleotide sequence capable of recognizing a signal-containing moiety, and a compound capable of binding to an insoluble phase.
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468. A composition of matter comprising:
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a first part which comprises more than one molecular bridging entity, each such entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, said analyte molecularly recognizable portion comprising a biological system selected from the group consisting of a virus or a viral component thereof and a cell or a cellular component thereof, said cell or cellular component thereof comprising a bacterium or a bacterial component thereof, said first portion being selected from the group consisting of an antigen, a polyclonal or a monoclonal antibody, a hormone, a receptor, an enzyme, an allosteric effector, an enzyme substrate, an enzyme cofactor, a protein and a protein receptor, and a second portion comprising one or more nucleic acid sequences or segments; and
a second part which comprises one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said more than one bridging entity nucleic acid second portion, and one or more signal generating portions capable of directly or indirectly providing a detectable signal, said direct signal providing signal generating portion being selected from the group consisting of a fluorogenic compound, a phosphorescent compound, a chromogenic compound, a chemiluminescent compound, an electron dense compound, an enzyme, and said indirect signal providing signal generating portion being selected from the group consisting of an antibody, an antigen, a hapten, a receptor, a ligand, an enzyme, a polynucleotide sequence capable of recognizing a signal-containing moiety, and a compound capable of binding to an insoluble phase.
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469. A composition of matter comprising:
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a first part which comprises an analyte having one or more molecularly recognizable portions thereon;
a second part which comprises more than one molecular bridging entity, each such entity comprising a first portion capable of recognizing and binding to or hybridizing with said molecularly recognizable analyte portion, said analyte molecularly recognizable portion comprising a biological system selected from the group consisting of d virus or a viral component thereof and a cell or a cellular component thereof, said cell or cellular component thereof comprising a bacterium or a bacterial component thereof, said first portion being selected from the group consisting of an antigen, a polyclonal or a monoclonal antibody, a hormone, a receptor, an enzyme, an allosteric effector, an enzyme substrate, an enzyme cofactor, a protein and a protein receptor, and a second portion comprising one or more nucleic acid sequences or segments; and
a third part which comprises one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said more than one bridging entity nucleic acid second portion, and one or more signal generating portions capable of directly or indirectly providing a detectable signal, said direct signal providing signal generating portion being selected from the group consisting of a fluorogenic compound, a phosphorescent compound, a chromogenic compound, a chemiluminescent compound, an electron dense compound, an enzyme, and said indirect signal providing signal generating portion being selected from the group consisting of an antibody, an antigen, a hapten, a receptor, a ligand, an enzyme, a polynucleotide sequence capable of recognizing a signal-containing moiety, and a compound capable of binding to an insoluble phase.
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470. A composition of matter comprising:
a complex which comprises;
more than one molecular bridging entity, each such entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, said analyte molecularly recognizable portion comprising a biological system selected from the group consisting of a virus or a viral component thereof and a cell or a cellular component thereof, said cell or cellular component thereof comprising a bacterium or a bacterial component thereof, said first portion being selected from the group consisting of an antigen, a polyclonal or a monoclonal antibody, a hormone, a receptor, an enzyme, an allosteric effector, an enzyme substrate, an enzyme cofactor, a protein and a protein receptor, and a second portion comprising one or more nucleic acid sequences or segments; and
one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said more than one bridging entity nucleic acid second portion, and one or more signal generating portions capable of directly or indirectly providing a detectable signal, said direct signal providing signal generating portion being selected from the group consisting of a fluorogenic compound, a phosphorescent compound, a chromogenic compound, a chemiluminescent compound, an electron dense compound, an enzyme, and said indirect signal providing signal generating portion being selected from the group consisting of an antibody, an antigen, a hapten, a receptor, a ligand, an enzyme, a polynucleotide sequence capable of recognizing a signal-containing moiety, and a compound capable of binding to an insoluble phase.
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471. A composition of matter comprising:
a complex which comprises;
an analyte having one or more molecularly recognizable portions thereon;
more than one molecular bridging entity, each such entity comprising a first portion capable of recognizing and binding to or hybridizing with said molecularly recognizable analyte portion, said analyte molecularly recognizable portion comprising a biological system selected from the group consisting of a virus or a viral component thereof and a cell or a cellular component thereof, said cell or cellular component thereof comprising a bacterium or a bacterial component thereof, said first portion being selected from the group consisting of an antigen, a polyclonal or a monoclonal antibody, a hormone, a receptor, an enzyme, an allosteric effector, an enzyme substrate, an enzyme cofactor, a protein and a protein receptor, and a second portion comprising one or more nucleic acid sequences or segments; and
one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said more than one bridging entity nucleic acid second portion, and one or more signal generating portions capable of directly or indirectly providing a detectable signal, said direct signal providing signal generating portion being selected from the group consisting of a fluorogenic compound, a phosphorescent compound, a chromogenic compound, a chemiluminescent compound, an electron dense compound, an enzyme, and said indirect signal providing signal generating portion being selected from the group consisting of an antibody, an antigen, a hapten, a receptor, a ligand, an enzyme, a polynucleotide sequence capable of recognizing a signal-containing moiety, and a compound capable of binding to an insoluble phase.
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472. A composition of matter comprising:
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a first part which comprises a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, said analyte molecularly recognizable portion comprising a biological system selected from the group consisting of a virus or a viral component thereof and a cell or a cellular component thereof, said cell or cellular component thereof comprising a bacterium or a bacterial component thereof, said first portion being selected from the group consisting of an antigen, a polyclonal or a monoclonal antibody, a hormone, a receptor, an enzyme, an allosteric effector, an enzyme substrate, an enzyme cofactor, a protein and a protein receptor, and a second portion comprising one or more nucleic acid sequences or segments, and a second part which comprises one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more chemically modified or artificially altered polynucleotides capable of directly or indirectly providing a detectable signal, said direct signal providing signal generating portion being selected from the group consisting of a fluorogenic compound, a phosphorescent compound, a chromogenic compound, a chemiluminescent compound, an electron dense compound, an enzyme, and said indirect signal providing signal generating portion being selected from the group consisting of an antibody, an antigen, a hapten, a receptor, a ligand, an enzyme, a polynucleotide sequence capable of recognizing a signal-containing moiety, and a compound capable of binding to an insoluble phase.
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473. A composition of matter comprising:
a complex which comprises;
a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, said analyte molecularly recognizable portion comprising a biological system selected from the group consisting of a virus or a viral component thereof and a cell or a cellular component thereof, said cell or cellular component thereof comprising a bacterium or a bacterial component thereof, said first portion being selected from the group consisting of an antigen, a polyclonal or a monoclonal antibody, a hormone, a receptor, an enzyme, an allosteric effector, an enzyme substrate, an enzyme cofactor, a protein and a protein receptor, and a second portion comprising one or more nucleic acid sequences or segments; and
one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more chemically modified or artificially altered polynucleotides capable of directly or indirectly providing a detectable signal, said direct signal providing signal generating portion being selected from the group consisting of a fluorogenic compound, a phosphorescent compound, a chromogenic compound, a chemiluminescent compound, an electron dense compound, an enzyme, and said indirect signal providing signal generating portion being selected from the group consisting of an antibody, an antigen, a hapten, a receptor, a ligand, an enzyme, a polynucleotide sequence capable of recognizing a signal-containing moiety, and a compound capable of binding to an insoluble phase.
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474. A composition of matter comprising:
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a first part which comprises an analyte having one or more molecularly recognizable portions thereon, said analyte molecularly recognizable portion comprising a biological system selected from the group consisting of a virus or a viral component thereof and a cell or a cellular component thereof, said cell or cellular component thereof comprising a bacterium or a bacterial component thereof;
a second part which comprises a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, said first portion being selected from the group consisting of an antigen, a polyclonal or a monoclonal antibody, a hormone, a receptor, an enzyme, an allosteric effector, an enzyme substrate, an enzyme cofactor, a protein and a protein receptor, and a second portion comprising one or more nucleic acid sequences or segments; and
a third part which comprises one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more chemically modified or artificially altered polynucleotides capable of directly or indirectly providing a detectable signal, said direct signal providing signal generating portion being selected from the group consisting of a fluorogenic compound, a phosphorescent compound, a chromogenic compound, a chemiluminescent compound, an electron dense compound, an enzyme, and said indirect signal providing signal generating portion being selected from the group consisting of an antibody, an antigen, a hapten, a receptor, a ligand, an enzyme, a polynucleotide sequence capable of recognizing a signal-containing moiety, and a compound capable of binding to an insoluble phase.
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475. A composition of matter comprising:
a complex which comprises;
an analyte having one or more molecularly recognizable portions thereon;
a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, said analyte molecularly recognizable portion comprising a biological system selected from the group consisting of a virus or a viral component thereof and a cell or a cellular component thereof, said cell or cellular component thereof comprising a bacterium or a bacterial component thereof, said first portion being selected from the group consisting of an antigen, a polyclonal or a monoclonal antibody, a hormone, a receptor, an enzyme, an allosteric effector, an enzyme substrate, an enzyme cofactor, a protein and a protein receptor, and a second portion comprising one or more nucleic acid sequences or segments; and
one or more non-radioactive signalling entities substantially incapable of binding to or hybridizing with the molecularly recognizable portion on said analyte, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more chemically modified or artificially altered polynucleotides capable of directly or indirectly providing a detectable signal, said direct signal providing signal generating portion being selected from the group consisting of a fluorogenic compound, a phosphorescent compound, a chromogenic compound, a chemiluminescent compound, an electron dense compound, an enzyme, and said indirect signal providing signal generating portion being selected from the group consisting of an antibody, an antigen, a hapten, a receptor, a ligand, an enzyme, a polynucleotide sequence capable of recognizing a signal-containing moiety, and a compound capable of binding to an insoluble phase.
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479. A kit for the detection in a sample of an analyte having one or more molecularly recognizable portions thereon, comprising as components thereof:
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(i) a container carrying a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with said molecularly recognizable portion on said analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
a container carrying more than one non-radioactive signalling entity, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity second portion nucleic acid sequence or segment, and one or more signal generating portions, each such portion being capable of providing a detectable signal;
wherein the ratio of the nucleic acid sequences or segments in the second portion to the first portion of the molecular bridging entity is selected from a number greater than 5 and a number greater than 10;
orwherein both the ratio of the nucleic acid sequences or segments in the second portion to the first portion of the molecular bridging entity and the ratio of the signal generating portions to the nucleic acid portion in any or all of the signalling entities is selected from a number greater than 1, a number greater than 5 and a number greater than 10. - View Dependent Claims (486)
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480. A kit for the detection in a sample of an analyte having one or more molecularly recognizable portions thereon, comprising as components thereof:
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a container carrying a complex which comprises;
a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with said molecularly recognizable portion, and a second portion comprising one or more nucleic acid sequences or segments; and
more than one non-radioactive signalling entity, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity second portion, and one or more signal generating portions, each such portion being capable of providing a detectable signal;
wherein the ratio of the nucleic acid sequences or segments in the second portion to the first portion of the molecular bridging entity is selected from a number greater than 5 and a number greater than 10;
orwherein both the ratio of the nucleic acid sequences or segments in the second portion to the first portion of the molecular bridging entity and the ratio of the signal generating portions to the nucleic acid portion in any or all of the signalling entities is selected from a number greater than 1, a number greater than 5 and a number greater than 10.
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481. A kit for the detection in a sample of an analyte having one or more molecularly recognizable portions thereon, comprising as components thereof:
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a container carrying more than one molecular bridging entity, each such entity comprising a first portion capable of recognizing and binding to or hybridizing with molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
a container carrying more than one non-radioactive signalling entity, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion to form a polynucleotide hybrid, and one or more signal generating portions capable of providing a detectable signal;
wherein the ratio of the nucleic acid sequences or segments in the second portion to the first portion of the molecular bridging entity is selected from a number greater than 5 and a number greater than 10;
orwherein both the ratio of the nucleic acid sequences or segments in the second portion to the first portion of the molecular bridging entity and the ratio of the signal generating portions to the nucleic acid portion in any or all of the signalling entities is selected from a number greater than 1, a number greater than 5 and a number greater than 10.
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482. A kit for the detection in a sample of an analyte having one or more molecularly recognizable portions thereon, comprising as components thereof:
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more than one molecular bridging entity, each such entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
more than one non-radioactive signalling entity, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal;
wherein the ratio of the nucleic acid sequences or segments in the second portion to the first portion of the molecular bridging entity is selected from a number greater than 5 and a number greater than 10;
orwherein both the ratio of the nucleic acid sequences or segments in the second portion to the first portion of the molecular bridging entity and the ratio of the signal generating portions to the nucleic acid portion in any or all of the signalling entities is selected from a number greater than 1, a number greater than 5 and a number greater than 10.
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483. A kit for the detection in a sample of an analyte having one or more molecularly recognizable portions thereon, comprising as components thereof:
a complex which comprises;
(i) more than one molecular bridging entity, each such entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
(ii) more than one non-radioactive signalling entity, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more signal generating portions capable of providing a detectable signal;
wherein the ratio of the nucleic acid sequences or segments in the second portion to the first portion of the molecular bridging entity is selected from a number greater than 5 and a number greater than 10;
orwherein both the ratio of the nucleic acid sequences or segments in the second portion to the first portion of the molecular bridging entity and the ratio of the signal generating portions to the nucleic acid portion in any or all of the signalling entities is selected from a number greater than 1, a number greater than 5 and a number greater than 10.
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484. A kit for the detection in a sample of an analyte having one or more molecularly recognizable portions thereon, comprising as components thereof:
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a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
more than one non-radioactive signalling entity, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion, and one or more polynucleotides which have been chemically modified or artificially altered;
wherein the ratio of the nucleic acid sequences or segments in the second portion to the first portion of the molecular bridging entity is selected from a number greater than 5 and a number greater than 10;
orwherein one or both the ratio of the nucleic acid sequences or segments in the second portion to the first portion of the molecular bridging entity and the ratio of the one or more chemically modified or artificially altered polynucleotides to the nucleic acid portion in any or all of the signalling entities is selected from a number greater than 1, a number greater than 5 and a number greater than 10. - View Dependent Claims (487)
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485. A kit for the detection in a sample of an analyte having one or more molecularly recognizable portions thereon, comprising as components thereof:
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a complex which comprises;
a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with a molecularly recognizable portion on an analyte, and a second portion comprising one or more nucleic acid sequences or segments; and
more than one non-radioactive signalling entity, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity nucleic acid second portion to form a polynucleotide hybrid, and one or more polynucleotides which have been chemically modified or artificially altered;
wherein the ratio of the nucleic acid sequences or segments in the second portion to the first portion of the molecular bridging entity is selected from a number greater than 5 and a number greater than 10;
orwherein one or both the ratio of the nucleic acid sequences or segments in the second portion to the first portion of the molecular bridging entity and the ratio of the one or more chemically modified or artificially altered polynucleotides to the nucleic acid portion in any or all of the signalling entities is selected from a number greater than 1, a number greater than 5 and a number greater than 10.
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506. (canceled)
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507. A polynucleotide sequence covalently attached to a monoclonal antibody.
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508. A polynucleotide sequence covalently attached to a lectin.
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509. (canceled)
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510. A polynucleotide sequence covalently attached to a hormone receptor.
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511. A polynucleotide sequence covalently attached to a hormone.
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512-527. -527. (canceled)
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528. A circular DNA molecule covalently attached to a non-radiolabeled signal generating moiety that comprises an enzyme.
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529. A circular DNA molecule comprising a polynucleotide that encodes part or all of a gene, wherein the DNA molecule is covalently attached to a non-radiolabeled signal generating moiety that comprises biotin.
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530. A circular DNA molecule comprising a polynucleotide that encodes part or all of a gene, wherein the DNA molecule is covalently attached to a non-radiolabeled signal generating moiety that comprises an antibody.
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531. A circular DNA molecule comprising a polynucleotide that encodes part or all of a gene, wherein the DNA molecule is covalently attached to a non-radiolabeled signal generating moiety that comprises a fluorogenic compound.
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546. A kit for the detection in a sample of an analyte having one or more molecularly recognizable portions thereon, comprising as components thereof:
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(i) a container carrying a molecular bridging entity comprising a first portion capable of recognizing and binding to or hybridizing with said molecularly recognizable portion on said analyte, and a second portion comprising one, or more nucleic acid sequences or segments; and
(ii) a container carrying more than one signalling entity, each such entity comprising a nucleic acid portion capable of hybridizing with said bridging entity second portion nucleic acid sequence or segment, and one or more signal generating portions, each such portion being capable of providing a detectable signal, wherein said analyte is a polynucleotide, said one or more nucleic acid sequences or segments of said second portion are repeating low complexity nucleic acid sequences or segments, and said one or more nucleic acid sequences or segments of said second portion are incapable of hybridizing to the analyte. - View Dependent Claims (547)
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548-552. -552. (canceled)
Specification
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Current AssigneeEnzo Biochem Incorporated
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Original AssigneeEnzo Biochem Incorporated
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InventorsRABBANI, ELAZAR, STAVRIANOPOULOS, JANNIS, ENGELHARDT, DEAN, KLINE, STAN, OLSIEWSKI, PAULA, PERGOLIZZI, ROBERT
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Granted Patent
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Time in Patent OfficeDays
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Field of Search
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US Class Current435/6
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CPC Class CodesC12Q 1/6813 Hybridisation assaysC12Q 1/6876 Nucleic acid products used ...G01N 33/53 Immunoassay; Biospecific bi...Y10T 436/143333 Saccharide [e.g., DNA, etc.]
