ADSORPTION COLUMN FOR PURIFYING BODY FLUID

• US 20100276359A1
• Filed: 09/08/2008
• Published: 11/04/2010
• Est. Priority Date: 09/12/2007
• Status: Abandoned Application

• Current Assignee: REI Medical Co., Ltd.
• Original Assignee: REI Medical Co., Ltd.
• Inventors: Nakanishi, Kazuki, Ishii, Sayaka, Kurusu, Chie, Miyamoto, Riichi, Minakuchi, Hiroyoshi, Ippommatsu, Masamichi, Yano, Keiko
• Application Number:
  US12/677,955
• Publication Number:
  US 20100276359A1
• Time in Patent Office: Days
• US Class Current: 210/446
• CPC Class Codes: A61M 1/3679 by absorption A61M1/3675 takes precedence
• CPC Class Codes: B01J 20/103 comprising silica
• CPC Class Codes: B01J 20/2808 being less than 2 nm, i.e. micropores or nanopores
• CPC Class Codes: B01J 20/28083 being in the range 2-50 nm, i.e. mesopores
• CPC Class Codes: B01J 20/28085 being more than 50 nm, i.e. macropores
• CPC Class Codes: B01J 20/28095 Shape or type of pores, voids, channels, ducts
• CPC Class Codes: B01J 20/3204 Inorganic carriers, supports or substrates
• CPC Class Codes: B01J 20/327 Polymers obtained by reactions involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3274 Proteins, nucleic acids, polysaccharides, antibodies or antigens
• CPC Class Codes: B01J 20/328 Polymers on the carrier being further modified
• CPC Class Codes: B01J 2220/58 Use in a single column

Abstract

Provided is an absorption column for body fluid purification by which the saturated adsorption amount of a substance to be adsorbed can be increased or the time required for reaching the saturated adsorption can be shortened, and therefore, the column capacity can be considerably reduced and the treatment time can be considerably shortened. The adsorption column having functional groups 6 specifically binding to a substance to be adsorbed including at least low density lipoprotein, immobilized on the surface of a porous support 5. The porous support 5 has double-pore structure comprising: a skeleton 7 made of a silica gel or silica glass having a three-dimensional network structure; through-pores 8 of an average diameter measured by the mercury intrusion method of 1 μm or more but less than 4 μm and having a three-dimensional network structure, which are formed in the voids of the skeleton; and micropores 9 having an average diameter measured by the mercury intrusion method larger than the grain diameter of the substance to be adsorbed, which are dispersedly formed on the surface of the skeleton.

Claims

1. An adsorption column for body fluid purification comprising

a porous support having a functional group immobilized on a surface of the porous support, the functional group specifically binding to a substance to be adsorbed including at least a low density lipoprotein, wherein

the porous support includes:

a skeleton made of a silica gel or silica glass having a three-dimensional network structure;

through-pores of an average diameter in a range of 1 μ

m or more to less than 4 μ

m as measured by a mercury intrusion method, the through-pores being formed in voids of the skeleton and having a three-dimensional network form; and

micropores of an average diameter of larger than a maximum length of the substance to be adsorbed in the body fluid purification as measured by a mercury intrusion method, the micropores penetrating from a surface of the skeleton to an inner part thereof and being dispersedly formed.

2. The adsorption column for body fluid purification according to claim 1, wherein

the average diameter of the micropores as measured by a mercury intrusion method is twice or more as large as the maximum length of the substance to be adsorbed.

3. The adsorption column for body fluid purification according to claim 1, wherein

the average diameter of the micropores as measured by a mercury intrusion method is 27 nm or more and 200 nm or less.

4. The adsorption column for body fluid purification according to claim 1, wherein

the functional group is dextran sulfate or a salt thereof, a polyacrylic acid or a salt thereof, or another chained polyhydric acid or a salt thereof having an affinity to specifically bind to a low density lipoprotein.

5. The adsorption column for body fluid purification according to claim 1, wherein

the porous support is synthesized by a spinodal decomposition sol-gel method.

6. The adsorption column for body fluid purification according to claim 1, wherein

the porous support is formed into a columnar shape and contained in a cylindrical container in such a way that a columnar lateral surface of the porous support is closely contacted with an inner wall surface of the cylindrical container, the cylindrical container having an opening at least in a part of each of both end surfaces, and

the opening in one end surface of the cylindrical container is communicated with the opening in other end surface thereof via the through-pores of the porous support.

7. The adsorption column for body fluid purification according to claim 1, wherein

the porous support is formed into a cylindrical shape and contained in a cylindrical container in such a way that at least a part of a cylindrical outer lateral surface of the porous support is spaced from an inner wall surface of the cylindrical container, the cylindrical container having an opening at least in a part of each of both end surfaces,

the opening in one end surface of the cylindrical container is communicated with both of an outer lateral part and an inner lateral part of the porous support, and

the opening in other end surface of the cylindrical container is communicated with either one of the outer lateral part and the inner lateral part of the porous support.