METHOD TO DETECT AND MONITOR ISCHEMIA IN TRANSPLANTED ORGANS AND TISSUES

• US 20100278738A1
• Filed: 05/04/2010
• Published: 11/04/2010
• Est. Priority Date: 05/04/2009
• Status: Abandoned Application

• Current Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Original Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Inventors: Sitzman, Thomas J., Gutowski, Karol A.
• Application Number:
  US12/773,389
• Publication Number:
  US 20100278738A1
• Time in Patent Office: Days
• US Class Current: 424/9.1
• CPC Class Codes: A61K 49/0008 Screening agents using (non-human) animal models or transgenic animal models or chimeric hosts, e.g. Alzheimer disease animal model, transgenic model for heart failure
• CPC Class Codes: A61P 9/10 for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis

Abstract

Disclosed is a method of detecting and/or monitoring ischemia in a tissue or organ, such as a free flap transfer. The method includes the steps of measuring in real time or near-real time interstitial glucose concentration, or rate of change of interstitial glucose concentration over time, or both, in the tissue or organ. A reduced glucose concentration or a negative rate of change of glucose concentration in the tissue or organ as compared to a control glucose concentration or rate of change indicates ischemia in the tissue or organ.

Claims

1. A method of detecting and/or monitoring ischemia in a tissue or organ, the method comprising:

measuring in real time or near-real time interstitial glucose concentration, or rate of change of interstitial glucose concentration over time, or both, in the tissue or organ, wherein a reduced glucose concentration or a negative rate of change of glucose concentration in the tissue or organ as compared to a control glucose concentration or rate of change indicates ischemia in the tissue or organ.

2. The method of claim 1, comprising measuring interstitial glucose concentration.

3. The method of claim 2, wherein a measured interstitial glucose concentration of less than about 118 mg/dL indicates ischemia in the tissue or organ.

4. The method of claim 1, comprising measuring rate of change of interstitial glucose concentration over time.

5. The method of claim 4, wherein a measured rate of change of interstitial glucose concentration steeper than about −

2 mg/dL/minute indicates ischemia in the tissue or organ.

6. The method of claim 4, wherein a measured rate of change of interstitial glucose concentration steeper than about −

3 mg/dL/minute indicates ischemia in the tissue or organ.

7. The method of claim 4, wherein a measured rate of change of interstitial glucose concentration steeper than about −

4 mg/dL/minute indicates ischemia in the tissue or organ.

8. The method of claim 1, comprising measuring interstitial glucose concentration and rate of change of interstitial glucose concentration over time.

9. The method of claim 8, wherein a measured interstitial glucose concentration of less than about 118 mg/dL and a measured rate of change of interstitial glucose concentration steeper than about −

2 mg/dL/minute indicates ischemia in the tissue or organ.

10. The method of claim 9, wherein a measured rate of change of interstitial glucose concentration steeper than about −

3 mg/dL/minute indicates ischemia in the tissue or organ.

11. The method of claim 9, wherein a measured rate of change of interstitial glucose concentration steeper than about −

4 mg/dL/minute indicates ischemia in the tissue or organ.

12. The method of claim 1, wherein the tissue or organ is an autologous free tissue transfer.

13. A method of detecting and/or monitoring ischemia in a free tissue transfer, the method comprising:

measuring in real time or near-real time interstitial glucose concentration, or rate of change of interstitial glucose concentration over time, or both, in the free tissue transfer, wherein a reduced glucose concentration or a negative rate of change of glucose concentration in the free tissue transfer as compared to a control glucose concentration or rate of change indicates ischemia in the free tissue transfer.

14. The method of claim 13, comprising measuring interstitial glucose concentration.

15. The method of claim 14, wherein a measured interstitial glucose concentration of less than about 118 mg/dL indicates ischemia in the free tissue transfer.

16. The method of claim 13, comprising measuring rate of change of interstitial glucose concentration over time.

17. The method of claim 16, wherein a measured rate of change of interstitial glucose concentration steeper than about −

2 mg/dL/minute indicates ischemia in the free tissue transfer.

18. The method of claim 17, wherein a measured rate of change of interstitial glucose concentration steeper than about −

3 mg/dL/minute indicates ischemia in the free tissue transfer.

19. The method of claim 17, wherein a measured rate of change of interstitial glucose concentration steeper than about −

4 mg/dL/minute indicates ischemia in the free tissue transfer.

20. The method of claim 13, comprising measuring interstitial glucose concentration and rate of change of interstitial glucose concentration over time.

21. The method of claim 20, wherein a measured interstitial glucose concentration of less than about 118 mg/dL and a measured rate of change of interstitial glucose concentration steeper than about −

2 mg/dL/minute indicates ischemia in the free tissue transfer.

22. The method of claim 21, wherein a measured rate of change of interstitial glucose concentration steeper than about −

3 mg/dL/minute indicates ischemia in the free tissue transfer.

23. The method of claim 21, wherein a measured rate of change of interstitial glucose concentration steeper than about −

4 mg/dL/minute indicates ischemia in the free tissue transfer.