ASSAY DEVICE AND METHOD
First Claim
1. A method of determining a sample component comprising:
- passing a fluid sample over a region surface of a microfluidic channel, the region comprising a maximum cross-sectional dimension of less than 1 mm;
allowing a sample component to bind with a binding partner disposed on the a surface of the region;
allowing a metal colloid to associate with the sample component;
flowing a metal solution over the surface to form a metallic layer; and
determining the metallic layer and thereby determining the sample component.
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Accused Products
Abstract
An assay method is described, which comprises the steps of immobilizing a binding partner (e.g., an antigen or antibody) for an analyte to be detected (e.g., an antibody or antigen) on a portion of a surface of a microfluidic chamber; passing a fluid sample over the surface and allowing the analyte to bind to the binding partner; allowing a metal colloid, e.g., a gold-conjugated antibody, to associate with the bound analyte; flowing a metal solution, e.g., a silver solution, over the surface such as to form an opaque metallic layer; and detecting the presence of said metallic layer, e.g., by visual inspection or by measuring light transmission through the layer, conductivity or resistance of the layer, or metal concentration in the metal solution after flowing the metal solution over the surface.
86 Citations
28 Claims
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1. A method of determining a sample component comprising:
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passing a fluid sample over a region surface of a microfluidic channel, the region comprising a maximum cross-sectional dimension of less than 1 mm; allowing a sample component to bind with a binding partner disposed on the a surface of the region; allowing a metal colloid to associate with the sample component; flowing a metal solution over the surface to form a metallic layer; and determining the metallic layer and thereby determining the sample component. - View Dependent Claims (2, 3, 4, 5, 6, 9, 10, 12, 13, 14, 15, 16, 17, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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7-8. -8. (canceled)
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11. (canceled)
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18. (canceled)
Specification