TEMPLATE-DEPENDENT NUCLEIC ACID POLYMERIZATION USING OLIGONUCLEOTIDE TRIPHOSPHATES BUILDING BLOCKS
First Claim
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1. A method of extending a nascent oligonucleotide-3′
- -OH in a template-dependent manner, the method comprising;
contacting the nascent oligonucleotide-3′
-OH with a nucleic acid template, a template-dependent polymerase and 4N oligonucleotide triphosphates each including N monomers, under conditions in which said nascent oligonucleotide-3′
-OH hybridizes with said nucleic acid template and said template-dependent polymerase is active in incorporating said oligonucleotide triphosphates onto a growing 3′
-OH group of the nascent oligonucleotide-3′
-OH, thereby extending the nascent oligonucleotide-3′
-OH in a template-dependent manner.
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Abstract
A novel use of a template-dependent polymerase. The novel use is effected by employing the template-dependent polymerase for incorporating at least one oligonucleotide triphosphate onto a nascent oligonucleotide-3′-OH in a template-dependent manner.
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Citations
1 Claim
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1. A method of extending a nascent oligonucleotide-3′
- -OH in a template-dependent manner, the method comprising;
contacting the nascent oligonucleotide-3′
-OH with a nucleic acid template, a template-dependent polymerase and 4N oligonucleotide triphosphates each including N monomers, under conditions in which said nascent oligonucleotide-3′
-OH hybridizes with said nucleic acid template and said template-dependent polymerase is active in incorporating said oligonucleotide triphosphates onto a growing 3′
-OH group of the nascent oligonucleotide-3′
-OH, thereby extending the nascent oligonucleotide-3′
-OH in a template-dependent manner.
- -OH in a template-dependent manner, the method comprising;
Specification
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Current AssigneeYeda Research and Development Co. Ltd. (Weizmann Institute of Science)
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Original AssigneeYeda Research and Development Co. Ltd. (Weizmann Institute of Science)
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InventorsKLESS, Hadar
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Application NumberUS12/838,512Publication NumberTime in Patent OfficeDaysField of SearchUS Class Current435/91.2CPC Class CodesC12P 19/34 Polynucleotides, e.g. nucle...C12Q 1/686 Polymerase chain reaction [...C12Q 2527/125 Specific component of sampl...
