COMPOSITIONS AND METHODS FOR QUANTIFICATION OF SERUM GLYCOPROTEINS

• US 20100279382A1
• Filed: 03/12/2010
• Published: 11/04/2010
• Est. Priority Date: 05/21/2004
• Status: Abandoned Application

• Current Assignee: Institute for System Biology
• Original Assignee: Institute for System Biology
• Inventors: Aebersold, Rudolf H., Zhang, Hui
• Application Number:
  US12/723,574
• Publication Number:
  US 20100279382A1
• Time in Patent Office: Days
• US Class Current: 435/200
• CPC Class Codes: G01N 2400/00 Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
• CPC Class Codes: G01N 2458/15 Non-radioactive isotope labels, e.g. for detection by mass spectrometry
• CPC Class Codes: G01N 33/6842 Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
• CPC Class Codes: G01N 33/6848 Methods of protein analysis involving mass spectrometry

Abstract

The invention provides compositions and methods for identifying and/or quantifying glycopolypeptides from human serum or plasma. The compositions and methods include a plurality of standard peptides containing glycosylation sites determined for human serum/plasma proteins.

Claims

1-40. -40. (canceled)

41. A composition comprising a plurality of peptides containing the glycosylation sites referenced as SEQ ID NOS:

1-3482, wherein said peptides each correspond to peptide fragments derived by cleavage of polypeptides using the same cleavage reagent.

42. The composition of claim 41, wherein said cleavage reagent is a protease.

43. The composition of claim 42, wherein said protease is trypsin.

44. A kit comprising a plurality of peptides containing the glycosylation sites sh referenced as SEQ ID NOS:

1-3482, wherein said peptides each correspond to peptide fragments derived by cleavage of polypeptides using the same cleavage reagent.

45. The kit of claim 44, further comprising a pair of differentially labeled isotope tags.

46. The kit of claim 44, further comprising the cleavage reagent corresponding to said peptide fragments.

47. The kit of claim 46, wherein said cleavage reagent is a protease.

48. The kit of claim 47, wherein said protease is trypsin.

49. The kit of claim 44, further comprising a hydrazide resin.

50. The kit of claim 44, further comprising a glycosidase.