APPLICATIONS OF UBIQUINONES AND UBIQUINOLS

• US 20100279413A1
• Filed: 04/30/2008
• Published: 11/04/2010
• Est. Priority Date: 01/14/2008
• Status: Abandoned Application

• Current Assignee: Zymes, LLC
• Original Assignee: Zymes, LLC
• Inventors: Fain, Randi
• Application Number:
  US12/598,400
• Publication Number:
  US 20100279413A1
• Time in Patent Office: Days
• US Class Current: 435/406
• CPC Class Codes: A61K 31/122 having the oxygen directly attached to a ring, e.g. quinones, vitamin K1, anthralin
• CPC Class Codes: C12N 2500/38 Vitamins
• CPC Class Codes: C12N 5/0018 Culture media for cell or tissue culture media for specific animal cell type C12N5/06

Abstract

The present invention relates to cell culture medium compositions comprising lipophilic compounds and solubilizing agent and methods of using such compositions for the culturing of cells. The invention also relates to methods of treating disorders of the nervous system such as peripheral neuropathy.

Claims

1. A water-soluble cell culture medium supplement composition, comprising:

(1) a ubiquinone or ubiquinol; and

(2) a solubilizing agent having a structure according to the following formula;

2. The composition according to claim 1, wherein said ubiquinone or ubiquinol has a structure which is a member selected from Formula (I) and Formula (II):

3. The composition according to claim 2, wherein said ubiquinone or ubiquinol is Coenzyme Q₁₀.

4. The composition according to claim 2, wherein said ubiquinone or ubiquinol is a Coenzyme Q₁₀ analog.

5. The composition according to claim 2, wherein said ubiquinone or ubiquinol is Coenzyme Q₁₀ and said solubilizing agent is PQS.

6. A culture medium composition, comprising:

a) a basal culture medium; and

b) water-soluble cell culture medium supplement composition according to claim 1.

7. The composition according to claim 6, wherein said basal medium is selected from the group consisting of RPMI 1640, Dulbecco'"'"'s modified Eagle'"'"'s Medium, and Ham'"'"'s F12.

8. A culture medium solution comprising:

a) a basal culture medium; and

b) water-soluble cell culture medium supplement composition according to claim 1, wherein said medium and said supplement composition are dissolved in water.

9. The solution according to claim 8, wherein said basal medium is selected from the group consisting of RPMI 1640, Dulbecco'"'"'s modified Eagle'"'"'s Medium, and Ham'"'"'s F12.

10. The solution according to claim 9, further comprises serum.

11. The solution according to claim 10, wherein said serum is fetal calf serum.

12. The solution according to claim 11, further comprises a growth factor.

13. The solution according to claim 12, wherein said growth factor is nerve growth factor.

14. A method of growing a cell, said method comprising the step of contacting said cells with a cell culture medium comprising:

a) a basal culture medium;

b) a ubiquinone or ubiquinol; and

c) a solubilizing agent having the structure according to the following formula;

15. The method according to claim 14, wherein said ubiquinone or ubiquinol has a structure which is a member selected from Formula (I) and Formula (II):

16. The composition according to claim 14, wherein said ubiquinone or ubiquinol is Coenzyme Q₁₀.

17. The composition according to claim 14, wherein said ubiquinone or ubiquinol is a Coenzyme Q₁₀ analog.

18. The composition according to claim 14, wherein said ubiquinone or ubiquinol is Coenzyme Q₁₀ and said solubilizing agent is PQS.

19. The method according to claim 14, wherein said cell is a neuron.

20. The method according to claim 14, wherein said cell is a glia.

21. The method according to claim 20, wherein said glia is a Schwann cell.

22. The method according to claim 14, wherein said cell is a primary cell culture cell.

23. The method according to claim 14, wherein said cell is derived from a cell line.

24. A method of culturing tissue or organ, said method comprising the step of contacting said tissue with a tissue culture medium comprising:

a) a basal culture medium;

b) a ubiquinone or ubiquinol; and

c) a solubilizing agent having the structure according to the following formula;

25. The method according to claim 24, wherein said ubiquinone or ubiquinol has a structure which is a member selected from Formula (I) and Formula (II):

26. The composition according to claim 24, wherein said ubiquinone or ubiquinol is Coenzyme Q₁₀.

27. The composition according to claim 24, wherein said ubiquinone or ubiquinol is a Coenzyme Q₁₀ analog.

28. The composition according to claim 24, wherein said ubiquinone or ubiquinol is Coenzyme Q₁₀ and said solubilizing agent is PQS.