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METHODS FOR NUCLEIC ACID MANIPULATION

  • US 20100285482A1
  • Filed: 05/24/2010
  • Published: 11/11/2010
  • Est. Priority Date: 04/20/2001
  • Status: Active Grant
First Claim
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1. A method for replicating and amplifying a target nucleic acid sequence comprising:

  • a) reacting a primer that is complementary to a target sequence within a nucleic acid duplex with the nucleic acid duplex in the presence of a recombination factor to form a recombination intermediate, without previously denaturing said nucleic acid duplex;

    b) admixing a polymerase with said recombination intermediate to form a polymerase complex, whereby the polymerase replicates the target sequence.

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