Apparatus and Method for High-Throughput Micro-Cell Culture with Mechanical Stimulation
First Claim
1. A method for high-throughput micro-cell culture with mechanical stimulation, comprising:
- providing cells on a membrane;
supplying a culture medium to the cells; and
vibrating the membrane by exerting and varying a fluid pressure on the membrane such that the cells are stimulated through the membrane.
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Accused Products
Abstract
A method for high-throughput micro-cell culture with mechanical stimulation includes providing cells on a membrane, supplying a culture medium to the cells, and vibrating the membrane by exerting and varying a fluid pressure on the membrane such that the cells are mechanically stimulated through the membrane.
An apparatus for high-throughput micro-cell culture with mechanical stimulation includes a cell culture vessel and a fluid pressure supply unit. The cell culture vessel has the membrane, a culture medium chamber, and a pressure chamber. The fluid pressure supply unit is connected fluidly to the pressure chamber, and has a fluid pressure supply device and a control device. The membrane vibrates when the fluid pressure in the pressure chamber is varied. The apparatus provides mechanical stimulation to the cultured cells in a controllable manner.
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Citations
16 Claims
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1. A method for high-throughput micro-cell culture with mechanical stimulation, comprising:
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providing cells on a membrane; supplying a culture medium to the cells; and vibrating the membrane by exerting and varying a fluid pressure on the membrane such that the cells are stimulated through the membrane. - View Dependent Claims (2, 3, 4, 5, 6)
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7. An apparatus for high-throughput micro-cell culture with mechanical stimulation, comprising:
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a cell culture vessel having a membrane that is thereinside adapted to support cells, a culture medium chamber that is disposed on one side of said membrane, and a pressure chamber that is disposed on the other side of said membrane and that has a fluid pressure transmittable to said membrane; and a fluid pressure supply unit connected fluidly to said pressure chamber, and having a fluid pressure supply device so as to supply a pressurized fluid to said pressure chamber, and a control device that is adapted to vary a pressure of the fluid supplied to said pressure chamber, thereby varying the fluid pressure, said membrane vibrating when the fluid pressure is varied. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification