PREANTRAL FOLLICLE DERIVED EMBRYONIC STEM CELLS
First Claim
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1. A method for producing a preantral follicle-derived embryonic stem cell, which comprises the steps of:
- (a) obtaining a preantral follicle from mammalian ovaries;
(b) growing the preantral follicle in vitro;
(c) maturing an oocyte in vitro present in the cultured preantral follicle;
(d) activating the matured oocyte for parthenogenesis;
(e) culturing the activated oocyte to form a blastocyst; and
(f) culturing inner cell mass (ICM) cells of the blastocyst to produce the preantral follicle-derived embryonic stem cell.
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Abstract
The present invention relates to a method for producing a preantral follicle-derived embryonic stem cell and a preantral follicle-derived embryonic stem cell. The present method comprises the steps of (a) obtaining a preantral follicle from mammalian ovaries; (b) growing the preantral follicle in vitro; (c) maturing an oocyte in vitro present in the cultured preantral follicle; (d) activating the matured oocyte for parthenogenesis; (e) culturing the activated oocyte to form a blastocyst; and (f) culturing inner cell mass (ICM) cells of the blastocyst to produce the preantral follicle-derived embryonic stem cell.
8 Citations
11 Claims
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1. A method for producing a preantral follicle-derived embryonic stem cell, which comprises the steps of:
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(a) obtaining a preantral follicle from mammalian ovaries; (b) growing the preantral follicle in vitro; (c) maturing an oocyte in vitro present in the cultured preantral follicle; (d) activating the matured oocyte for parthenogenesis; (e) culturing the activated oocyte to form a blastocyst; and (f) culturing inner cell mass (ICM) cells of the blastocyst to produce the preantral follicle-derived embryonic stem cell. - View Dependent Claims (2, 3, 4, 5, 6, 7, 11)
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- 8. A preantral follicle-derived embryonic stem cell, wherein the embryonic stem cell has the same karyotype as an oocyte present in the preantral follicle, is stainable with alkaline phosphatase and capable of forming an embryonic body and teratoma.
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10. (canceled)
Specification