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METHODS OF SEQUENCING NUCLEIC ACIDS

  • US 20100285970A1
  • Filed: 03/31/2010
  • Published: 11/11/2010
  • Est. Priority Date: 03/31/2009
  • Status: Abandoned Application
First Claim
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1. A method of sequencing nucleic acid, comprising:

  • a) preparing single stranded fragments of a first nucleic acid having a substantially known sequence, wherein each of the fragments has a substantially known sequence;

    b) preparing single stranded fragments of a second nucleic acid having an unknown sequence;

    c) contacting the single stranded fragments of a) or amplification products (copies) thereof, and the single stranded fragments of b) or copies thereof under conditions that allow formation of heterohybrid nucleic acid, wherein the heterohybrid nucleic acid comprises perfectly complementary heterohybrid nucleic acid and heterohybrid nucleic acid containing a mismatch;

    d) distinguishing formation of heterohybrid nucleic acid containing a mismatch from formation of heterohybrid nucleic acid which is perfectly complementary; and

    e) determining sequences of the mismatches in d), thus allowing elucidation of the sequence of the second nucleic acid.

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