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METHOD FOR MAKING ANTIBODY FC-HETERODIMERIC MOLECULES USING ELECTROSTATIC STEERING EFFECTS

  • US 20100286374A1
  • Filed: 01/06/2009
  • Published: 11/11/2010
  • Est. Priority Date: 01/07/2008
  • Status: Active Grant
First Claim
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1. A method of preparing a heterodimeric protein comprising a first CH3-containing polypeptide and a second CH3-containing polypeptide that meet together to form an interface engineered to promote heterodimer formation, wherein said first CH3-containing polypeptide and said second CH3-containing polypeptide comprise one or more charged amino acids within the interface that are electrostatically unfavorable to homodimer formation but electrostatically favorable to heterodimer formation, the method comprising the steps of:

  • (a) culturing a host cell comprising a nucleic acid encoding the first CH3-containing polypeptide and a nucleic acid comprising the second CH3-containing polypeptide, wherein the cultured host cell expresses the first and second CH3-containing polypeptides; and

    (b) recovering the heterodimeric protein from the host cell culture.

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