IMMUNOGENICITY USING A COMBINATION OF DNA AND VACCINIA VIRUS VECTOR VACCINES
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Abstract
This invention relates to improved methods of inducing an immune response for the prevention or treatment of HIV-1 infection by using a nucleic acid vaccine in conjunction with a recombinant viral vaccine, e.g., a poxvirus vaccine, to potentiate and broaden the immune response. The present invention further provides a particularly effective vaccine regimen comprising a DNA vaccine used in combination with a poxvirus virus, especially NYVAC or ALVAC.
35 Citations
27 Claims
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1-14. -14. (canceled)
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15. A method of potentiating a CD8+ response to human immunodeficiency virus-1 (HIV-1) epitopes in a human comprising:
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administering an expression vector encoding HIV-1 Gag, Pol, Pro, Tat, Nef, Rev, Vif, Vpr or Env antigens; and administering a recombinant Modified Vaccinia virus Ankara (MVA) encoding the same antigens encoded by the expression vector; wherein the expression vector and the recombinant MVA enter the cells of the human and intracellularly produce HIV peptides that are presented on the cell'"'"'s MHC class I molecules in an amount sufficient to stimulate a CD8+ response, and further, wherein administration of the combination of the expression vector and the recombinant MVA potentiates the immune response compared to administration of either the expression vector or the recombinant MVA by itself. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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27. A method of reducing viral load in a mammal that is infected with an immunodeficiency virus, comprising:
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administering to the mammal an expression vector that expresses encoded immunodeficiency virus Gag, Pol and Env antigens; and later administering to the mammal a recombinant MVA encoding said antigens; wherein the expression vector and the recombinant MVA enter the cells of the mammal and intracellularly produce said antigens, and wherein administration of the combination of the expression vector and the recombinant MVA reduces the viral load more than administration of either the expression vector or the recombinant MVA by itself.
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Specification