Modification of CXCR4 using engineered zinc finger proteins
First Claim
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1. A zinc finger DNA-binding domain that binds to a target site as shown in Table 1 in a CXCR4 gene.
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Abstract
Disclosed herein are methods and compositions for modulating activity of CXCR4 genes, for example using zinc finger transcription factors (ZF-TFs) or zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding ZF-TFs or ZFNs, vectors comprising polynucleotides encoding ZF-TFs or ZFNs and cells comprising polynucleotides encoding ZF-TFs or ZFNs and/or cells comprising ZF-TF or ZFNs are also provided.
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Citations
19 Claims
- 1. A zinc finger DNA-binding domain that binds to a target site as shown in Table 1 in a CXCR4 gene.
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9. A method of inactivating an endogenous cellular CXCR4 gene in a cell, the method comprising:
(a) introducing, into a cell, a first nucleic acid encoding a first polypeptide, wherein the first polypeptide comprises; (i) a zinc finger DNA-binding domain that is engineered to bind to a first target site in an endogenous CXCR4 gene; and (ii) a cleavage domain;
such that the polypeptide is expressed in the cell, whereby the polypeptide binds to the target site and cleaves the CXCR4 gene.- View Dependent Claims (10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
Specification