NUCLEIC ACID STRAND USEFUL IN DETECTING SUBSTANCE AND METHOD THEREOF
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Accused Products
Abstract
To detect an infinitesimal substance with a high sensitivity without amplifying the infinitesimal substance. A nucleic acid strand for detection P having at least a fluorescent substance F which can serve as a donor of resonance excitation energy (fluorescence resonance energy), a quencher substance Q which is located at a position enabling it to receive the resonance excitation energy, and a nucleic acid strand region N which is located between the quencher substance Q and the fluorescent substance F and has an enzyme cleavage site X to be cleaved by an endonuclease; and a detection technique using the nucleic acid strand for detection P.
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Citations
16 Claims
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1-8. -8. (canceled)
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9. A nucleic acid strand for detection comprising:
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a fluorescent substance which can serve as a donor of resonance excitation energy including fluorescence resonance energy; a quencher substance which is located at a position enabling it to receive the resonance excitation energy; and a nucleic acid strand region which is located between the quencher substance and the fluorescent substance and has an enzyme cleavage site to be cleaved by an endonuclease formed therein. - View Dependent Claims (10, 11, 12, 13)
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14. A method using a nucleic acid strand comprising:
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allowing a complementary strand formation reaction involved in a nucleic acid strand for detection having at least a fluorescent substance which can serve as a donor of resonance excitation energy including fluorescence resonance energy, a quencher substance which is located at a position enabling it to receive the resonance excitation energy, and a nucleic acid strand region which is located between the quencher substance and the fluorescent substance and has an enzyme cleavage site to be cleaved by an endonuclease to proceed; and cleaving a probe nucleic acid strand at the enzyme cleavage site by allowing the endonuclease specific for a double strand obtained by the complementary strand formation reaction to act thereon to effect fragmentation and also dissociating the cleaved strand into single strands thereby amplifying the fluorescence of the fluorescent substance. - View Dependent Claims (15, 16)
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Specification