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Molecular diagnostics system and methods

  • US 20100291666A1
  • Filed: 12/21/2005
  • Published: 11/18/2010
  • Est. Priority Date: 12/23/2004
  • Status: Active Grant
First Claim
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1. An integrated single-use device for performing a nucleic acid analysis, comprising:

  • a housing,an entry port for accepting a sample suspected of containing a target nucleic acid, a first chamber operably connected to the entry port containing a reagent for extracting the target nucleic acid,a first conduit permitting passage of extracted nucleic acid into an amplification chamber, said housing containing an amplification reagent that is capable of incorporating a detectable label into an amplified nucleic acid target,the amplification chamber having a heating means and a temperature sensing means for controlling amplification conditions,the amplification chamber being operably linked to a second conduit containing a sensing region with an immobilized capture oligonucleotide,said housing containing a means for moving the amplified target to the sensing region to permit binding of said amplified target to said capture oligonucleotide, said second conduit operably attached to a holding chamber containing a fluid able to substantially displace uncaptured amplified target from the sensing region and permitting sensing of said detectable label retained in said sensing region.

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