METHOD OF MEASURING GLYCATED HEMOGLOBIN CONCENTRATION AND CONCENTRATION MEASURING APPARATUS

• US 20100291691A1
• Filed: 03/23/2007
• Published: 11/18/2010
• Est. Priority Date: 03/24/2006
• Status: Active Grant

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Sugiyama, Koji, Sakai, Toshikatsu
• Granted Patent: US 8,268,625 B2
• Time in Patent Office: Days
• US Class Current: 436/67
• CPC Class Codes: G01N 2030/8822 involving blood
• CPC Class Codes: G01N 21/31 Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry G01N21/72 takes precedence
• CPC Class Codes: G01N 30/74 Optical detectors measurement of intensity, velocity, spectral content, polarisation, or phase of infrared, visible or ultraviolet light G01J
• CPC Class Codes: G01N 30/8679 Target compound analysis, i.e. whereby a limited number of peaks is analysed
• CPC Class Codes: G01N 30/88 Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86

Abstract

When the concentration of glycated hemoglobin is measured, a plurality of wavelengths are selected as measurement wavelengths from the wavelength range of 400 to 450 nm. Preferably, by use of a liquid chromatography, at least the light of different peak wavelengths in the wavelength range of 415 to 430 nm are continuously or intermittently received to obtain a three dimensional chromatogram having as variables the wavelength, the elution time and the amount of detection. The concentration of glycated hemoglobin is calculated based on this three dimensional chromatogram.

Claims

1. A method of measuring the concentration of glycated hemoglobin based on light that travels from a sample when the sample is irradiated with light, wherein

the concentration of the glycated hemoglobin is measured based on light of a plurality of measurement wavelengths having a peak wavelength in the wavelength range of 400 to 450 nm.

2. The method of measuring the concentration of glycated hemoglobin according to claim 1, wherein

the concentration of glycated hemoglobin is measured by continuously or intermittently receiving light of different peak wavelengths in the wavelength range of at least 415 to 430 nm among the light that travels from a sample.

3. The method of measuring the concentration of glycated hemoglobin according to claim 1, wherein

in the method of measuring the concentration of glycated hemoglobin using a liquid chromatography, the concentration of the glycated hemoglobin is calculated based on a three dimensional chromatogram in which the measurement wavelength, the elution time and the amount of detection are made variables.

4. The method of measuring the concentration of glycated hemoglobin according to claim 3, wherein

the concentration of glycated hemoglobin is calculated as a proportion occupied by the volume or integrated value corresponding to the glycated hemoglobin in the volume or integrated value corresponding to the total amount of hemoglobin in the three dimensional chromatogram.

5. The method of measuring the concentration of glycated hemoglobin according to claim 3, wherein

the concentration of glycated hemoglobin is obtained by calculation of the proportion of the glycated hemoglobin from a chromatogram in which the elution time and the amount of detection in each measurement wavelength are made variables and also by averaging of the proportion of the glycated hemoglobin in each measurement wavelength.

6. The method of measuring the concentration of glycated hemoglobin according to claim 3, wherein

the concentration of glycated hemoglobin can is calculated as a proportion occupied by an area corresponding to glycated hemoglobin in an area corresponding to the total amount of hemoglobin in a two dimensional chromatogram with the peak value and elution time of the detection value of hemoglobin in each measurement wavelength.

7. The method of measuring the concentration of glycated hemoglobin according to claim 1, wherein

the concentration of glycated hemoglobin is measured based on a first quantity of light that is an amount of light having a peak wavelength in the wavelength range of 400 to 420 nm, traveling from a sample, and a second quantity of light that is an amount of light having a peak wavelength in the wavelength range of 420 to 440 nm, traveling from the sample.

8. The method of measuring the concentration of glycated hemoglobin according to claim 7, wherein

the concentration of hemoglobin is obtained by calculating the concentration of oxyhemoglobin or a value that correlates to this concentration based on the first quantity of light on the one hand, and by calculating the concentration of deoxyhemoglobin or a value that correlates to this concentration based on the second quantity of light on the other, and

by adding up the oxyhemoglobin concentration and the deoxyhemoglobin concentration or by adding up a value that correlates to the oxyhemoglobin concentration and a value that correlates to the deoxyhemoglobin concentration.

9. The method of measuring the concentration of glycated hemoglobin according to claim 7, wherein

in a method of measuring the concentration of glycated hemoglobin making use of liquid chromatography, the concentration of glycated hemoglobin is calculated based on a chromatogram produced by overlapping a first chromatogram that corresponds to the oxyhemoglobin indicating the relationship between the elution time and the amount of detection obtained based on the first quantity of light and a second chromatogram that corresponds to the deoxyhemoglobin indicating the relationship between the elution time and the amount of detection obtained based on the second quantity of light.

10. The method of measuring the concentration of glycated hemoglobin according to claim 1, wherein

the sample is produced by hemolyzing a blood cell.

11. An apparatus of measuring the concentration of glycated hemoglobin, comprising a photometry mechanism in which a sample is irradiated with light from a light source and at the time alight receiving section receives light that travels from the sample, wherein

the photometry mechanism is configured to distinguish light of a plurality of wavelengths having a peak wavelength in the wavelength range of 400 to 450 nm and be capable of receiving light in the light receiving section.

12. The apparatus of measuring the concentration of glycated hemoglobin according to claim 11, wherein

the light receiving section is configured to be continuously or intermittently able to receive light of different peak wavelengths in the wavelength range of at least 415 to 430 nm.

13. The apparatus of measuring the concentration of glycated hemoglobin according to claim 11, further comprising:

in an apparatus of measuring the concentration of glycated hemoglobin making use of liquid chromatography, a calculating section configured to calculate the glycated hemoglobin concentration based on a three dimensional chromatogram in which the wavelength, the elution time and the amount of detection are made variables.

14. The apparatus of measuring the concentration of glycated hemoglobin according to claim 13, wherein

the calculating section is configured to calculate the concentration of glycated hemoglobin as a proportion occupied by a volume or integrated value corresponding to the glycated hemoglobin in a volume or integrated value corresponding to the total amount of hemoglobin in the three dimensional chromatogram.

15. The apparatus of measuring the concentration of glycated hemoglobin according to claim 13, wherein

the calculating section is configured to calculate the proportion of the glycated hemoglobin from a chromatogram in which the elution time and the amount of detection in each measurement wavelength are made variables and also to calculate the glycated hemoglobin concentration by averaging the proportion of the glycated hemoglobin in each measurement wavelength.

16. The apparatus of measuring the concentration of glycated hemoglobin according to claim 13, wherein

the calculating section is configured to calculate as a proportion occupied by an area corresponding to glycated hemoglobin in an area corresponding to the total amount of hemoglobin in a two dimensional chromatogram with the peak value and elution time of the detection value of hemoglobin in each peak wavelength.

17. The apparatus of measuring the concentration of glycated hemoglobin according to claim 11, further comprising:

a calculating section of calculating the glycated hemoglobin concentration based on a first quantity of light that is an amount of light having a peak wavelength in the wavelength range of 400 to 420 nm, traveling from a sample, and a second quantity of light that is an amount of light having a peak wavelength in the wavelength range of 420 to 440 nm, traveling from the sample.

18. The apparatus of measuring the concentration of glycated hemoglobin according to claim 17, wherein

the calculating section is configured to calculate the concentration of oxyhemoglobin or a value that correlates to this concentration based on the first quantity of light on the one hand, and to calculate the concentration of deoxyhemoglobin or a value that correlates to this concentration based on the second quantity of light on the other, and also to add up the oxyhemoglobin concentration and the deoxyhemoglobin concentration or to add up a value that correlates to the oxyhemoglobin concentration and a value that correlates to the deoxyhemoglobin concentration to thereby calculate the concentration of the glycated hemoglobin.

19. The apparatus of measuring the concentration of glycated hemoglobin according to claim 17, wherein

in an apparatus of measuring the concentration of glycated hemoglobin making use of liquid chromatography, the calculating section is configured to calculate the concentration of glycated hemoglobin based on a chromatogram produced by overlapping a first chromatogram that corresponds to the oxyhemoglobin indicating the relationship between the elution time and the amount of detection obtained based on the first quantity of light and a second chromatogram that corresponds to the deoxyhemoglobin indicating the relationship between the elution time and the amount of detection obtained based on the second quantity of light.

20. The apparatus of measuring the concentration of glycated hemoglobin according to claim 11, wherein

the sample is produced by hemolyzing a blood cell.