CONTROL OF PLANT GENE EXPRESSION

• US 20100293664A1
• Filed: 04/03/2008
• Published: 11/18/2010
• Est. Priority Date: 04/03/2007
• Status: Abandoned Application

• Current Assignee: Vialactia Biosciences (Nz) Limited
• Original Assignee: Vialactia Biosciences (Nz) Limited
• Inventors: Phillips, Jonathan Robert, Withana, Nimali Piyushika, Templeton, Kerry Robert, Bajaj, Shivendra, Smith-Espinoza, Claudia Jeannette, Bryant, Catherine Jane, Puthigae, Sathish
• Application Number:
  US12/594,585
• Publication Number:
  US 20100293664A1
• Time in Patent Office: Days
• US Class Current: 800/278
• CPC Class Codes: C12N 15/8222 Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation

Abstract

This invention provides an isolated promoter polynucleotide comprising the sequence of SEQ ID NO:1, and variants and fragments thereof capable of controlling transcription of an operably linked polynucleotide in a plant. The invention also provides the constructs, vectors, host cells, plant cells and plants transformed with the isolated promoter polynucleotide. The invention also provides methods of use of the isolated promoter polynucleotide.

Claims

1-18. -18. (canceled)

19. An isolated promoter polynucleotide comprising at least one of:

a) the sequence of SEQ ID NO;

1;

b) a sequence with at least 70% identity to the sequence of SEQ ID NO;

1;

c) a sequence fragment consisting of at least 50 contiguous nucleotides of the sequence of SEQ ID NO;

1;

d) a sequence fragment consisting of at least 50 contiguous nucleotides of the sequence

of b);

e) a sequence with at least 70% identity to the sequence of c); and

f) a sequence that is a complement of any one of a) to e)

wherein the promoter polynucleotide is capable of controlling transcription of an operably linked polynucleotide in a plant.

20. The isolated promoter polynucleotide of claim 19, comprising a sequence with at least 70% identity to the sequence of SEQ ID NO:

1.

21. The isolated promoter polynucleotide of claim 19, comprising a sequence fragment consisting of at least 50 contiguous nucleotides of the sequence of

SEQ ID NO:

1.

22. The isolated promoter polynucleotide of claim 19, comprising a sequence fragment consisting of a sequence selected from any one of SEQ ID NO:

10 to SEQ ID NO;

16.

23. The isolated promoter polynucleotide of claim 19, comprising the sequence of SEQ ID NO:

1.

24. The isolated promoter polynucleotide of claim 19, consisting of the sequence of SEQ ID NO:

1.

25. The isolated promoter polynucleotide of claim 19, which is capable of controlling transcription of an operably linked polynucleotide sequence in a plant in at least one of leaves, internodes, roots and flowers.

26. The isolated promoter polynucleotide of claim 19, which is capable of controlling transcription of an operably linked polynucleotide sequence constitutively in substantially all tissues of a plant.

27. A genetic construct comprising a promoter polynucleotide of claim 19.

28. The genetic construct comprising a promoter polynucleotide of claim 19, in which the promoter polynucleotide is operably linked to a polynucleotide sequence to be expressed.

29. A plant cell or plant transformed with the promoter polynucleotide of claim 19.

30. A plant cell or plant transformed with a genetic construct comprising a promoter polynucleotide of claim 19.

31. A method for producing a plant cell or plant with modified expression of at least one polynucleotide, the method comprising:

(a) transforming plant cell or plant with a promoter polynucleotide of claim 19, and

(b) the cultivating the transgenic plant cell or plant under conditions conducive for the promoter polynucleotide to drive transcription.

32. A method for producing a plant cell or plant with modified expression of at least one polynucleotide, the method comprising:

(a) transforming plant cell or plant with a genetic construct comprising a promoter polynucleotide of claim 19, and

(b) the cultivating the transgenic plant cell or plant under conditions conducive for the promoter polynucleotide to drive transcription.

33. A method for producing a plant cell or plant with a modified phenotype, the method including the stable incorporation into the genome of the plant, a promoter polynucleotide of claim 19.

34. A method for producing a plant cell or plant with a modified phenotype, the method including the stable incorporation into the genome of the plant, a genetic construct comprising a promoter polynucleotide of claim 19.

35. A seed, propagule, progeny or part of a plant transformed with the promoter polynucleotide of claim 19, wherein the seed, propagule, progeny or part is transformed with the polynucleotide.

36. A seed, propagule, progeny or part of a plant transformed with a genetic construct comprising a promoter polynucleotide of claim 19, wherein the seed, propagule, progeny or part is transformed with the genetic construct.