PLURIPOTENT STEM CELLS CHARACTERIZED BY EXPRESSION OF GERMLINE SPECIFIC GENES
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Abstract
The present invention relates to pluripotent or multipotent somatic stem cells characterized by the expression of the germline specific gene, DAZL. The somatic stem cells expressing the DAZL marker are further characterized by expression of additional markers and absence of expression of certain blood markers. In particular, the present invention discloses therapeutic and diagnostic uses, other than the germ cell potential use, of the DAZL pluripotent or multipotent stem cells isolated from adult or peripheral somatic sources such as peripheral blood, bone marrow or umbilical cord blood.
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Citations
57 Claims
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1-37. -37. (canceled)
- 38. An isolated somatic multipotent or pluripotent stem cell characterized by expression of the germline specific gene DAZL and at least one gene selected from the group consisting of SOX-2, OCT-4, c-Kit and Stellar.
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51. A method of isolating pluripotent or multipotent somatic cells from an adult or perinatal source which comprises:
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separating a mononuclear cell fraction from a cell population; introducing a molecular probe targeting the specific marker DAZL and optionally at least one additional marker selecting from the group consisting of Sox-2, Stellar, Oct-4 and c-kit into the mononuclear cell fraction thus obtained; sorting the cells by means of sorting methodology; and isolating the stem cells expressing the specific marker DAZL and optionally the at least one of the genes selected from group consisting of Sox-2, Stellar, Oct-4 and c-kit. - View Dependent Claims (52, 53, 54)
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55. A kit for isolation, enrichment and detection of multipotent or pluripotent stem cells within a specimen, comprising:
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at least one reagent to detect DAZL protein or DAZL RNA; optionally reagents to detect at least one of the genes or gene products selected from the group consisting of;
Sox-2, Stellar, C-kit, and Oct-4;instructions for labeling, sorting and enrichment of the cells; and
optionallymeans for performing stem cell labeling, sorting and enrichment. - View Dependent Claims (56, 57)
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Specification