OSCILLATING CELL CULTURE BIOREACTOR
First Claim
1. A bioreactor comprisingOne or more gas permeable, closed-loop chambers for cell or tissue culture;
- a tissue engineering scaffold secured within the gas permeable, closed-loop chamber;
a means for introducing or removing fluid and gases into/from the closed-loop chamber;
an oscillating means for moving the gas permeable, closed-loop chamber bidirectionally along an axis horizontal to an axis normal to the closed-loop chamber to force cells and fluid within the gas permeable, closed-loop chamber through the secured tissue engineering scaffold.
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Accused Products
Abstract
Methods and devices for cell or tissue culture are provided. One aspect provides a bioreactor having a gas permeable, closed-loop chamber for cell or tissue culture, and an oscillating means for moving the gas permeable, closed-loop chamber bidirectionally along an axis horizontal to an axis normal to the closed-loop chamber to force convection of cells and fluid in the gas permeable, closed-loop chamber. The bioreactor optionally includes a tissue engineering scaffold, an inlet means, an outlet means, and integrated sensors. Another aspect provides a bioreactor having a plurality of gas permeable, closed-loop chambers for cell or tissue culture. Methods of culturing cells and producing tissue constructs are also provided.
125 Citations
17 Claims
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1. A bioreactor comprising
One or more gas permeable, closed-loop chambers for cell or tissue culture; -
a tissue engineering scaffold secured within the gas permeable, closed-loop chamber; a means for introducing or removing fluid and gases into/from the closed-loop chamber; an oscillating means for moving the gas permeable, closed-loop chamber bidirectionally along an axis horizontal to an axis normal to the closed-loop chamber to force cells and fluid within the gas permeable, closed-loop chamber through the secured tissue engineering scaffold. - View Dependent Claims (2, 3, 4, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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- 6. The bioreactor of claim 6, wherein the housing oxygenates and buffers the pH of a fluid in the gas permeable, closed-loop chamber.
Specification