AUTOMATIC DETECTION OF INFECTIOUS DISEASES

• US 20100297645A1
• Filed: 10/22/2008
• Published: 11/25/2010
• Est. Priority Date: 10/29/2007
• Status: Abandoned Application

• Current Assignee: Biocartis NV (Biocartis Group NV)
• Original Assignee: Biocartis SA (Biocartis Group NV)
• Inventors: Dijksman, Johan F., Pierik, Anke
• Application Number:
  US12/739,556
• Publication Number:
  US 20100297645A1
• Time in Patent Office: Days
• US Class Current: 435/6.1
• CPC Class Codes: B01L 1/52 Transportable laboratories; Field kits
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12Q 1/6888 for detection or identification of organisms

Abstract

The invention relates to the detection of infectious diseases. A system for automatic detection of infectious diseases is disclosed. The system comprises an input unit for receiving a blood sample, a lysis unit, a PCR unit, a sample unit and a detection unit. The systemmay based on an inputted blood sample generate an output signal that isindicative of the presence of pathogen DNA in the blood sample.

Claims

1. A system for automatic detection of infectious diseases, the system comprises:

an input unit (2) for receiving a blood sample;

a lysis unit (3) separating DNA content from the received blood sample to a separation sample;

a PCR unit (6) for receiving the separation sample and multiplying a number of gene sequences in the separation sample to provide a target sample with target molecules;

a sample unit (8) for receiving the target sample, and contacting the gene sequences with a substrate (20) having immobilized thereon probe molecules that specifically binds the target molecules;

a detection unit (9) for detecting the presence of resultant binding complexes on the substrate to determine the presence of the target molecules in the blood sample;

wherein an output signal is generated indicative of the presence of target molecules.

2. The system according to claim 1, wherein the input unit is connected to a catheter (14) for receiving the blood sample.

3. The system according to claim 1, further comprising or being connected to a decision support system, wherein the output signal is inputted into the decision support system.

4. The system according to claim 3, wherein a signal indicative of physiological information is inputted into the decision support system.

5. The system according to claim 3, wherein the decision support system outputs a signal, based on the received inputs, to a medicament administration unit (13).

6. The system according to claim 1, wherein the input unit provides a continuous flow of the blood sample to the lysis unit or provides a predefined volume of blood at predefined time intervals.

7. The system according to claim 1, wherein the PCR unit is operated in accordance with an unsaturated PCR process or a saturated PCR process.

8. The system according to claim 1, wherein the target molecules are provided with fluorophores.

9. The system according to claim 8, wherein the presence of the target molecules is detected by detecting luminescence of the target.

10. The system according to claim 9, wherein the presence of the target molecule is detected by detecting changes in luminescence of the target.

11. The system according to claim 1, wherein the number of gene sequences is a number of gene sequences of predefined bacteria DNA, funghi DNA or viral DNA.

12. A method of operating a system for automatic detection of infectious diseases, the method comprises:

receiving (30) a blood sample;

separating (31) DNA content from the received blood sample by lysis to provide a separation sample;

multiplying (33) a number of gene sequences in the separation sample by PCR to provide a target sample with target molecules;

contacting (34) the target sample with a substrate having immobilized thereon probe molecules that specifically binds the target molecules;

detecting (35) the presence of resultant binding complexes on the substrate to determine the presence of the target molecules in the blood sample; and

generating (36) a signal indicative of the presence of target molecules.

13. A computer program product having a set of instructions, when in use on a computer, causes a system for automatic detection of infectious diseases to perform the steps of claim 12.