MOLECULAR DIAGNOSTICS SYSTEM AND METHODS
First Claim
Patent Images
1. A nucleic acid separation method, comprising:
- exposing a sample comprising cells containing nucleic acid to an aqueous mixture comprising a lytic reagent and one or more beads capable of binding the nucleic acid released from said cells to form a nucleic acid-bead complex; and
passing the nucleic acid-bead complex through a substantially immiscible liquid layer to separate the nucleic acid from the aqueous mixture.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention relates to automated devices and methods for the extraction of nucleic acids from cells, the amplification of segments of nucleic acid and the detection of nucleic acids, all in a convenient and portable manner. The invention is particularly suited for use in point-of-care medical diagnostics testing.
95 Citations
56 Claims
-
1. A nucleic acid separation method, comprising:
-
exposing a sample comprising cells containing nucleic acid to an aqueous mixture comprising a lytic reagent and one or more beads capable of binding the nucleic acid released from said cells to form a nucleic acid-bead complex; and passing the nucleic acid-bead complex through a substantially immiscible liquid layer to separate the nucleic acid from the aqueous mixture. - View Dependent Claims (2, 3, 4, 5, 6, 7)
-
-
8. A method of transferring nucleic acid, comprising:
-
contacting nucleic acid at a first location with one or more beads to form a nucleic acid-bead complex in a liquid; and transporting the nucleic acid-bead complex to a second location separated from the first location by an intermediary layer, wherein said intermediary layer is substantially immiscible with the liquid. - View Dependent Claims (9)
-
-
10. A nucleic acid separation method, comprising:
-
introducing a sample comprising biological cells through a first layer to a second layer comprising magnetic beads, wherein said first layer is substantially contiguous with said second layer; incubating the sample for sufficient time to permit lysis of the cells and form a nucleic acid-bead complex in said second layer; and applying a magnetic field in proximity to the complex sufficient to move said complex from said second layer through said first layer, thereby effectively filtering said complex. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
-
-
32. A method for extracting and amplifying nucleic acid, comprising:
-
introducing a sample comprising biological cells through a first layer to a second layer comprising magnetic beads, wherein said first layer is substantially contiguous with said second layer; incubating the sample for sufficient time to permit lysis of the cells and form a nucleic acid-bead complex in said second layer; applying a magnetic field in proximity to the complex sufficient to move said complex from said second layer through said first layer, thereby substantially removing said complex from said second and first layers; and introducing said complex to a vessel containing a polymerase chain reaction (PCR) cocktail, wherein at least a portion of the nucleic acid elutes off said magnetic beads during a first heating cycle of the PCR. - View Dependent Claims (33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53)
-
-
54. A method for extracting nucleic acid from a biological sample, comprising:
-
applying a biological sample comprising cells containing nucleic acid to a biochemically inert filter positioned between a first location and a second location in a conduit; retaining the sample on the filter for sufficient time to permit extraction of nucleic acid onto the filter; applying a wash fluid through the conduit, whereby said wash fluid substantially removes from the filter matter capable of interfering with a nucleic acid amplification reaction, while substantially retaining the extracted nucleic acid on the filter; and applying an aqueous solution to the filter at a temperature above about 75°
C. to elute the extracted nucleic acid from the filter. - View Dependent Claims (55, 56)
-
Specification