COENZYME-BINDING GLUCOSE DEHYDROGENASE

• US 20100297743A1
• Filed: 08/06/2010
• Published: 11/25/2010
• Est. Priority Date: 12/24/2002
• Status: Abandoned Application

• Current Assignee: PHC Corporation (PHC Holdings Corporation)
• Original Assignee: PHC Corporation (PHC Holdings Corporation)
• Inventors: Kano, Kenji, Morita, Tetsunari, Sanada, Hirokazu, Ikeda, Tokuji, Kuyama, Mika, Tsujimura, Seiya, Omura, Hironori, Yada, Takako
• Application Number:
  US12/851,668
• Publication Number:
  US 20100297743A1
• Time in Patent Office: Days
• US Class Current: 435/287.1
• CPC Class Codes: C12N 9/0004 Oxidoreductases (1.)
• CPC Class Codes: C12Q 1/32 involving dehydrogenase
• CPC Class Codes: C12Q 1/54 involving glucose or galactose

Abstract

The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.

Claims

1-21. -21. (canceled)

22. A biosensor for measuring glucose, comprising soluble flavin compound-binding glucose dehydrogenase, which:

(a) catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor; and

(b) has a 5% or less specific activity to maltose relative to the enzymatic activity to glucose.