Multi-Channel Bioreactor with the Immobillization of Optical Sensing Membrane

• US 20100297744A1
• Filed: 12/26/2007
• Published: 11/25/2010
• Est. Priority Date: 08/08/2007
• Status: Abandoned Application

• Current Assignee: Industry Foundation Of Chonnam National University (Chonnam National University)
• Original Assignee: Industry Foundation Of Chonnam National University (Chonnam National University)
• Inventors: Rhee, Jong IL, Sohn, Ok-Jae
• Application Number:
  US12/671,978
• Publication Number:
  US 20100297744A1
• Time in Patent Office: Days
• US Class Current: 435/287.2
• CPC Class Codes: B01L 2300/046 Function or devices integrated in the closure
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 2021/7796 Special mountings, packaging of indicators
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/783 for analysing gases
• CPC Class Codes: G01N 21/80 Indicating pH value
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: G01N 33/588 with semiconductor nanocrystal label, e.g. quantum dots
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH

Abstract

The present invention relates to a multi-channel microbioreactor. More specifically, the present invention relates to a multi-channel microbioreactor having a number of wells in which an optical sensing membrane comprising a fluorescent dye and a bioconjugate, or a sensor material including a biomolecule conjugated to a fluorescent dye and a bioconjugate is formed. The multi-channel microbioreactor according to the present invention can be used for an in-situ optical detection of dissolved oxygen, carbon dioxide, pH, monosaccharides, polysaccharides, organic acids, alcohols, cholesterol, choline and xanthine, etc.

Claims

1. A multi-channel mini bioreactor having a number of wells in which an optical sensing membrane comprising a fluorescent dye and a bioconjugate, or a sensor material including a biomolecule conjugated to a fluorescent dye and a bioconjugate, is formed.

2. The multi-channel mini bioreactor of claim 1, wherein said wells are formed and aligned in a single frame.

3. The multi-channel mini bioreactor of claim 2, wherein the optical sensing membrane, which is a single membrane or divided into number n, is individually formed depending on each well, with n being an integer between 1 and 100.

4. The multi-channel mini bioreactor of claim 3, wherein said optical sensing membrane contains a single or more than one sensor material, that is adsorbed, bonded covalently, or captured by nano particles and micro particles.

5. The multi-channel mini bioreactor of claim 4, wherein said optical sensing membrane is formed at the bottom of a well.

6. The multi-channel mini bioreactor of claim 1, wherein said fluorescent dye is selected from the group consisting of a ruthenium complex, HPTS (8-hydroxypyrene-1,3,6-trisulfonate trisodium salt) and fluorescein amine.

7. The multi-channel mini bioreactor of claim 6, wherein said fluorescent dye detects dissolved oxygen, carbon dioxide and pH optically.

8. The multi-channel mini bioreactor of claim 6, wherein the hydrophobic polymer membrane is coated on top of the optical sensing membrane comprising said HPTS in order to achieve a selective detection of dissolved carbon dioxide.

9. The multi-channel mini bioreactor of claim 8, wherein said hydrophobic polymer membrane is selected from the group consisting of a silicone resin, PMMA and vernice, or a mixture thereof.

10. The multi-channel mini bioreactor of claim 1, wherein quantum dots having a CdSe core that is coated with a ZnS shell are coated with a hydrophilic surfactant and then conjugated with oxidases or oxidases and peroxidases in said bioconjugate.

11. The multi-channel mini bioreactor of claim 10, wherein said oxidases are selected from the group consisting of glucose oxidase, lactate oxidase, tyramine oxidase, ascorbic acid oxidase, and xanthine oxidase, or a mixture thereof.

12. The multi-channel mini bioreactor of claim 1, wherein said biomolecule is protein, amino acid, enzyme, antigen and antibody.

13. The multi-channel mini bioreactor of claim 1, wherein said optical sensing membrane immobilizes the sensor material by using a sol-gel method.

14. The multi-channel mini bioreactor of claim 13, wherein said sol-gel method immobilizes the sensor material by using alkoxysilane selected from the group consisting of 3-glycidoxypropyltrimethoxysilane (GPTMS), methyltriethoxysilane (MTES), aminopropyltrimethoxysilane (APTMS), phenyltrimethoxysilane (PTMS), and methyltrimethoxysilane (MTMS), or a mixture thereof.

15. The multi-channel mini bioreactor of claim 1, wherein a baffle board is installed on the cover of said wells.

16. The multi-channel mini bioreactor of claim 15, wherein said baffle board increases a stirring effect, an oxygen-transferring speed and a material-transferring speed.

17. The multi-channel mini bioreactor of claim 1, wherein a transparent polymer film coated with an optical sensing membrane is immobilized on said wells using an adhesive.

18. The multi-channel mini bioreactor of claim 17, wherein said optical sensing membrane can be divided into n sections and each divided membrane can achieve an individual optical detection, with n being an integer between 1 and 100.

19. A multi-channel optical detection system prepared by using the multi-channel mini bioreactor of claim 1.