INTEGRATED FLUIDIC CIRCUITS

• US 20100297748A1
• Filed: 03/26/2010
• Published: 11/25/2010
• Est. Priority Date: 09/28/2006
• Status: Abandoned Application

• Current Assignee: Stokes Bio Ltd. (Perspecta, Inc.), Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Stokes Bio Ltd. (Perspecta, Inc.)
• Inventors: Davies, Mark, Dalton, Tara
• Application Number:
  US12/732,877
• Publication Number:
  US 20100297748A1
• Time in Patent Office: Days
• US Class Current: 435/289.1
• CPC Class Codes: B01F 33/3021 the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
• CPC Class Codes: B01F 35/712 for feeding fluids
• CPC Class Codes: B01F 35/7172 using capillary forces
• CPC Class Codes: B01F 35/71805 using valves, gates, orifices or openings
• CPC Class Codes: B01L 2200/146 Employing pressure sensors
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/14 Means for pressure control
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/502784 specially adapted for droplet or plug flow, e.g. digital microfluidics automatic analysis using a stream of discrete samples in a tube system G01N35/08
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046

Abstract

The invention generally relates to an integrated fluidic circuit. In certain embodiments, the circuit includes a main chamber having a withdrawal port, a carrier fluid occupying a volume in the chamber, in which the carrier fluid is immiscible with a sample fluid, and a plurality of liquid bridges disposed within the chamber.

Claims

1. An integrated fluidic circuit comprising:

a main chamber having a withdrawal port;

a carrier fluid occupying a volume in the chamber, wherein the carrier fluid is immiscible with a sample fluid; and

a plurality of liquid bridges disposed within the chamber.

2. The circuit according to claim 1, wherein the main chamber further comprises a supply port that delivers the carrier fluid to the chamber.

3. The circuit according to claim 1, wherein each liquid bridge comprises:

at least one inlet in liquid communication with the main chamber for introducing at least one sample fluid into the main chamber; and

at least one outlet in liquid communication with the main chamber, wherein the outlet is separated from the inlet such that the sample fluid forms a droplet wrapped in the carrier fluid prior to entering the outlet.

4. The circuit according to claim 3, wherein each liquid bridge further comprises an auxiliary chamber having a withdrawal port in liquid communication with the main chamber, the auxiliary chamber housing a distal portion of the inlet and a proximal portion of the outlet.

5. The circuit according to claim 1, wherein an air bubble is present within the circuit.

6. The circuit according to claim 3, wherein the inlet and the outlet are of different lengths and different inner diameters.

7. The circuit according to claim 1, wherein the carrier fluid is oil.

8. The circuit according to claim 3, wherein the at least one inlet is two inlets.

9. The circuit according to claim 8, wherein a first inlet delivers a first sample fluid to the bridge, a second inlet delivers a second sample fluid to the bridge, and the outlet receives a droplet comprising a mixture of the first sample fluid and the second sample fluid wrapped in the carrier fluid.

10. The circuit according to claim 9, wherein the first inlet and the outlet are co-axial, and the second inlet is substantially perpendicular to the axis.

11. The circuit according to claim 9, wherein the second inlet has a smaller cross-sectional area than the first inlet.

12. The circuit according to claim 9, wherein a first inlet delivers the sample fluid to the bridge, and the second inlet delivers the carrier fluid to the bridge.

13. The circuit according to claim 3, wherein the at least one inlet is three inlets.

14. The circuit according to claim 13, wherein a first inlet delivers a first sample fluid to the bridge, a second inlet delivers a second sample fluid to the bridge, a third inlet delivers the carrier fluid to the bridge, and the outlet receives a droplet comprising a mixture of the first sample fluid and the second sample fluid wrapped in the carrier fluid.

15. The circuit according to claim 14, wherein a first sample fluid and a second sample fluid mix at the bridge to form mixed droplets wrapped in carrier fluid that are received by the outlet.

16. The circuit according to claim 15, wherein the withdrawal port of the main chamber withdraws the carrier fluid from between the first and second sample fluids before the first and second sample fluids bridge to the outlet of each liquid bridge so that the first and second sample fluids are caused to mix at each bridge.

17. The circuit according to claim 9, wherein the first sample fluid and the second sample fluid comprise different chemical species within an aqueous phase.

18. The circuit according to claim 9, wherein the first sample fluid comprises genetic material and the second sample fluid comprises PCR reagents.

19. The circuit according to claim 1, further comprising a flow controller.

20. The circuit according to claim 1, wherein the circuit is fluidly connected to a thermocycler and a detection module for analyzing contents of the droplet.

21. A microfluidic circuit comprising:

a chamber having a withdrawal port;

a carrier fluid occupying a volume in the chamber;

a plurality of inlets in liquid communication with the chamber for introducing at least one sample fluid into the chamber, wherein the sample fluid is immiscible with the carrier fluid; and

a plurality of outlets in liquid communication with the chamber, wherein each outlet has at least one corresponding inlet, and the outlet is separated from the inlet such that the sample fluid forms a droplet wrapped in the carrier fluid prior to entering the outlet.