BIOARRAY CHIP REACTION APPARATUS AND ITS MANUFACTURE
First Claim
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1. An apparatus for detecting an analyte in a test sample comprising:
- at least one probe array, wherein the at least one probe array comprises;
a plurality of particles having biological polymers immobilized on the plurality of particles thereon;
a slide, wherein the plurality of particles are attached to a surface of the slide; and
a barcode for identifying the at least one probe array on the slide.
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Abstract
A body 300 having a cavity 310 for mounting a substrate 120 fabricated with probe sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, is provided. The cavity includes inlets 350 and 360 for introducing selected fluids into the cavity to contact the probes. Accordingly, a commercially feasible device for use in high throughput assay systems is provided.
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Citations
35 Claims
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1. An apparatus for detecting an analyte in a test sample comprising:
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at least one probe array, wherein the at least one probe array comprises; a plurality of particles having biological polymers immobilized on the plurality of particles thereon; a slide, wherein the plurality of particles are attached to a surface of the slide; and a barcode for identifying the at least one probe array on the slide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A package for supporting a probe array, comprising:
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a plurality of particles having biological polymers immobilized on the plurality of particles thereon; a slide, wherein the plurality of particles are attached to a surface of the slide; and a top piece and a bottom piece, wherein the top bottom pieces house the first wafer, creating at least one cavity such that at least one probe array is in fluid communication with the reaction chamber. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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24. A method of using a probe array, comprising:
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providing at least one probe array, wherein the at least one probe array comprises; a plurality of particles having biological polymers immobilized on the plurality of particles thereon and a slide, wherein the plurality of particles are attached to a surface of the slide; providing a housing, wherein the housing comprises a cavity to accommodate a fluid to be in contact with the slide; placing the slide into the housing; sealably mounting the slide within the housing comprising a mounting surface and a fluid cavity, wherein the fluid cavity comprises at least one inlet port constructed to permit fluid flow into the cavity through the at least one inlet port, wherein the sealably mounting act provides the at least one probe array located inside the cavity; introducing the fluid inside the cavity to hybridize the at least one probe array on the slide; performing hybridization, wherein at least some of the polymers hybridizes to a target; aligning the at least one probe array, wherein the at least one probe array comprises at least one alignment feature; and detecting a signal from the at least one probe array using a scanner. - View Dependent Claims (25, 26, 27, 28, 29, 30)
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31. An apparatus for mixing a fluid, the apparatus comprising:
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a pump, wherein a first housing comprises the pump; a probe array; a substrate, wherein the substrate and the probe array create at least one cavity for a fluid; at least one bubble disposed within said chamber, wherein the at least one bubble is moved by using the pump to move the bubble relative to the fluid to effect mixing the fluid. - View Dependent Claims (32, 33, 34)
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35. A fluidic station comprising:
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a base that is adapted to provide a framework for the fluidics station; a mounting system coupled to the base, wherein the mounting system is movable between a loading position and a closed position, the mounting system comprising; a bottom piece adapted to receive a wafer, wherein a wafer comprises at least one probe array, wherein the bottom piece is adapted to receive a top piece positioned above the wafer forming at least one cavity for fluid to be in contact with the wafer and forming a package comprising at least an inlet port and an outlet port for fluid transfer and connecting with at least one cavity; at least one needle coupled to the base frame, wherein the needle includes a distal end and side ports above the distal ends; and at least one fluid source in communication with the at least one needle, wherein the wafer is insertable into the mounting system when in the loading position, and wherein the at least one needle is adapted to transfer liquid into the package through the liquid channels when in the closed position.
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Specification