PRODUCTION OF FERMENTIVE END PRODUCTSFROM CLOSTRIDIUM sp.
First Claim
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1. A method for producing a fermentive end-product comprising:
- culturing a medium comprising Clostridium for a first period of time under conditions suitable for production of a fermentive end-product by said;
adding one or more nutrients to the medium comprising Clostridium while prior to harvesting the fermentive end product;
culturing a medium comprising Clostridium for a second period of time; and
harvesting a fermentive end-product from the medium.
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Abstract
In one aspect, methods to enhance the production of ethanol and other fermentive end products from a wide variety of feedstocks by Clostridium microorganisms, such as Clostridium phytofermentans are disclosed. A method of improving fermentation performance of Clostridium microorganisms, such as Clostridium phytofermentans through the use of a fed-batch strategy is described, as well as methods of producing fermentive end products, such as alcohols and/or chemicals by fermenting Clostridium microorganisms, such as Clostridium phytofermentans in the presence of fatty acid-containing compounds and/or at reduced pH.
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Citations
47 Claims
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1. A method for producing a fermentive end-product comprising:
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culturing a medium comprising Clostridium for a first period of time under conditions suitable for production of a fermentive end-product by said; adding one or more nutrients to the medium comprising Clostridium while prior to harvesting the fermentive end product; culturing a medium comprising Clostridium for a second period of time; and harvesting a fermentive end-product from the medium. - View Dependent Claims (2, 3, 4, 5)
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6. A method of producing a fermentive end product comprising the steps of:
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culturing a strain of Clostridium phytofermentans in a medium; maintaining the total concentration of sugar compounds in the medium at least about 18 g/L; and harvesting a fermentive end-product from the medium. - View Dependent Claims (7, 8, 9, 10, 11, 12, 13, 14)
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15. A method of producing a fermentive end product, the method comprising the steps of:
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culturing a strain of Clostridium in a medium; and adding one or more medium components to the medium during the culturing of the Clostridium wherein one or more of the medium components comprises one or more sugar compounds, and the one or more sugar compounds are added in relation to an amount of sugar converted by the Clostridium to other compounds. - View Dependent Claims (16, 17, 18, 19, 21, 22)
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20. A method of producing a fermentive end product, the method comprising:
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adding a first inoculum of a strain of Clostridium to a medium; culturing the Clostridium under conditions suitable for production of ethanol; adding additional viable cells of Clostridium sp. to the medium more than five hours after the first inoculum of Clostridium is added to the medium; and harvesting the fermentive end product from the medium.
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23. A method of producing ethanol, the method comprising the steps of:
removing an impurity from an impure ethanol material to produce a purified ethanol material, wherein the purified ethanol material is more than about 90% (wt.) ethanol, and the impure ethanol material is derived from a fermentation medium made by culturing Clostridium phytofermentans cells in a fed batch culture, and wherein the ethanol concentration in the fermentation medium is greater than about 7 g/L.
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24. A method of producing a fermentive end product, the method comprising the steps of:
- culturing a medium comprising a strain of Clostridium phytofermentans, wherein the fermentive end product is produced at an instantaneous productivity of at least about 3 g/L-day.
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25. A method of producing a fermentive end product, comprising:
providing a cellulosic material, wherein said cellulosic material has not been treated with exogenously supplied chemicals or enzymes;
combining the cellulosic material with a microbe in a medium, wherein the medium does not comprise exogenously supplied enzymes; and
fermenting the cellulosic material under conditions and for a time sufficient to produce a fermentive end product.
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26. A method of producing a fermentive end product, the method comprising:
- fermenting cells of Clostridium phytofermentans in the presence of a pH modifier, wherein a fermentive end product is produced.
- View Dependent Claims (27, 28, 29)
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30. A method of producing a fermentive end product, the method comprising:
- fermenting cells of a Clostridium strain in the presence of an added fatty acid material, wherein a fermentive end product is produced.
- View Dependent Claims (31, 32)
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33. A fermentation medium, the medium comprising cells of Clostridium phytofermentans and a pH modifier, wherein a fermentive end product is produced.
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34. A fermentation medium, the medium comprising cells of a Clostridium strain and an added fatty acid containing compound, wherein a fermentive end product is produced.
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35. A fermentation medium comprising a strain of Clostridium phytofermentans, a nitrogen source comprising proline, glycine, histidine, and/or isoleucine, and a cellulosic or lignocellulosic material.
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36. A method of producing alcohol, the method comprising:
- fermenting cells of a Clostridium strain and the presence of a pH modifier and a fatty acid material, wherein a fermentive end product is produced.
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37. A fuel plant comprising a fermenter configured to house a medium and a strain of Clostridium phytofermentans, wherein said fermenter is configured to maintain an amount of sugar compounds at a level that varies by less than about 25% during fermentation.
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38. A fuel plant comprising a fermenter configured to house a medium and a strain of Clostridium phytofermentans, wherein said fermenter is configured to periodically supplement said medium with additional medium components or additional viable cells of Clostridium phytofermentans.
- 39. A fuel plant comprising a fermenter configured to house a medium and a strain of Clostridium phytofermentans, wherein said medium comprises a pH modifier and a cellulosic or lignocellulosic material.
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41. A fuel plant comprising a fermenter configured to house a medium and a strain of Clostridium phytofermentans, wherein said medium comprises a nitrogen source comprising proline, glycine, histidine, and/or isoleucine, and a cellulosic or lignocellulosic material.
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42. A fuel plant comprising a fermenter configured to house a medium and a strain of Clostridium phytofermentans, wherein said medium comprises a fatty acid material and a cellulosic or lignocellulosic material.
- 43. A fermentive end product produced by fermenting a cellulosic or lignocellulosic material with a strain of Clostridium phytofermentans, in a medium comprising an amount of sugar compounds at a level that varies by less than about 25% during fermentation.
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44. A fermentive end product produced by fermenting a cellulosic or lignocellulosic material with a strain of Clostridium phytofermentans, in a medium comprising a pH modifier.
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45. A fermentive end product produced by fermenting a cellulosic or lignocellulosic material with a strain of Clostridium phytofermentans, in a medium comprising a fatty acid.
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46. A fermentive end product produced by fermenting a cellulosic or lignocellulosic material with a strain of Clostridium phytofermentans, in a medium comprising a nitrogen source comprising proline, glycine, histidine, and/or isoleucine.
Specification