METHOD FOR ANALYZING ANALYTE IN TISSUE FLUID, ANALYZER FOR ANALYZING ANALYTE IN TISSUE FLUID, CARTRIDGE FOR ANALYZING ANALYTE IN TISSUE FLUID, AND KIT FOR ANALYZING ANALYTE IN TISSUE FLUID

• US 20100298671A1
• Filed: 05/25/2010
• Published: 11/25/2010
• Est. Priority Date: 05/25/2009
• Status: Abandoned Application

• Current Assignee: Sysmex Corporation
• Original Assignee: Sysmex Corporation
• Inventors: Kojima, Junko, Sato, Toshiyuki, Asakura, Yoshihiro
• Application Number:
  US12/786,704
• Publication Number:
  US 20100298671A1
• Time in Patent Office: Days
• US Class Current: 600/309
• CPC Class Codes: A61B 10/0045 Devices for taking samples of body liquids devices for taking blood samples A61B5/15
• CPC Class Codes: A61B 2010/008 Interstitial fluid
• CPC Class Codes: A61B 2562/0295 Strip shaped analyte sensors for apparatus classified in A61B5/145 or A61B5/157
• CPC Class Codes: A61B 5/1451 for interstitial fluid
• CPC Class Codes: A61B 5/14514 using means for aiding extraction of interstitial fluid, e.g. microneedles or suction
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement

Abstract

A method for analyzing an analyte in tissue fluid is disclosed. The method comprises: placing a collecting member in contact with the skin of a subject, wherein the collecting member is configured to collect and retain tissue fluid containing the analyte from the subject; collecting tissue fluid from the subject into the collecting member; detaching the collecting member from the skin of the subject; causing the analyte in the tissue fluid collected by the collecting member to diffuse in a liquid by supplying the collecting member with the liquid; and detecting the analyte diffused in the liquid.

An analyzer for analyzing an analyte in tissue fluid, and cartridge and kit for use in the analyzing method are also disclosed.

Claims

1. A method for analyzing an analyte in tissue fluid, comprising:

placing a collecting member in contact with a skin of a subject, wherein the collecting member is configured to collect and retain tissue fluid containing an analyte from the subject;

collecting tissue fluid from the subject into the collecting member;

detaching the collecting member from the skin of the subject;

causing the analyte in the tissue fluid collected by the collecting member to diffuse in a liquid by supplying the collecting member with the liquid; and

detecting the analyte diffused in the liquid.

2. An analyzing apparatus for analyzing an analyte in tissue fluid, comprising:

an accommodating part configured to accommodate a collecting member containing tissue fluid collected from a subject;

a reservoir configured to retain liquid, wherein the reservoir is arranged in communication with the accommodating part;

a liquid transporter configured to transport liquid to the accommodating part and to transport liquid to the reservoir from the accommodating part; and

a detector for detecting an analyte in the liquid transported into the reservoir by the liquid transporter.

3. The analyzing apparatus according to claim 2, further comprising:

a cartridge comprising the accommodating part and the reservoir; and

a cartridge holder configured to detachably hold the cartridge.

4. The analyzing apparatus according to claim 3, wherein

the cartridge comprises a reagent.

5. The analyzing apparatus according to claim 4, wherein

the reagent causes a change in optical characteristics by reacting with the analyte in the liquid retained in the reservoir;

and

the detector is configured to detect the analyte based on the change in the optical characteristics of the reagent.

6. The analyzing apparatus according to claim 2, wherein

the detector comprises an electrode configured to contact the liquid retained in the reservoir.

7. The analyzing apparatus according to claim 2, wherein

the reservoir comprises a first reservoir and a second reservoir;

and

the detector comprises a first detector for detecting a first analyte in the liquid retained in the first reservoir, and a second detector for detecting a second analyte in the liquid retained in the second reservoir.

8. The analyzing apparatus according to claim 7, wherein

the accommodating part and the first reservoir are connected via a flow path;

the second reservoir is disposed in the flow path; and

the first reservoir comprises a reagent.

9. The analyzing apparatus according to claim 8, wherein

the first detector is disposed opposite a first surface of the cartridge set in the cartridge holder;

and

the second detector is disposed opposite a second surface of the cartridge set in the cartridge holder.

10. The analyzing apparatus according to claim 3, wherein

the cartridge has a channel that communicates with the accommodating part and the reservoir when the cartridge is set in the cartridge holder.

11. The analyzing apparatus according to claim 2, wherein

the liquid transporter is configured to move the liquid in the accommodating part to the reservoir by supplying air to the accommodating part.

12. The analyzing apparatus according to claim 7, further comprising

a controller for performing analysis relating to the first analyte based on the first analyte detection results obtained by the first detector and the second analyte detection results obtained by the second detector.

13. A cartridge configured to be detachably installed in an analyzing apparatus for analyzing an analyte in tissue fluid, the cartridge comprising:

an accommodating part configured to accommodate liquid supplied from the apparatus and a collecting member containing tissue fluid collected from a subject so that the collecting member is in contact with the liquid; and

a reservoir arranged in communication with the accommodating part, wherein the reservoir is configured to retain liquid transferred from the accommodating part.

14. The cartridge according to claim 13, further comprises

a reagent.

15. The cartridge according to claim 13, wherein

the reservoir comprises a first reservoir that communicates with the accommodating part and allows detection of the first analyte by the analyzing apparatus, and a second reservoir that communicates with the first reservoir and allows detection of the second analyte by the analyzing apparatus.

16. The cartridge according to claim 15, wherein

the accommodating part and the first reservoir are connected via a flow path;

the second reservoir is disposed in the flow path; and

the first reservoir comprises a reagent.

17. The cartridge according to claim 13, wherein

the cartridge further comprises a channel that communicates with the accommodating part and the reservoir when the cartridge is placed in the analyzing apparatus.

18. An analyzing kit for analyzing an analyte in tissue fluid, comprising:

a collecting member for collecting and retaining tissue fluid collected from a subject; and

the cartridge according to claim 13.

19. The kit according to claim 18, wherein the collecting member comprises:

a collecting body for collecting and retaining tissue fluid collected from a subject; and

an adhesive film for holding the collecting body;

wherein

the collecting body is accommodated in the accommodating part by adhering the adhesive film to the margin of the opening when the collecting body is inserted through the opening of the accommodating part.

20. The kit according to claim 19, wherein

the collecting body is a gel.

21. The kit according to claim 19, wherein

the adhesive film is of sufficient size to be capable of closing the opening of the accommodating part.