METHODS AND COMPOSITIONS FOR SELECTING AN IMPROVED PLANT

• US 20100299773A1
• Filed: 05/12/2010
• Published: 11/25/2010
• Est. Priority Date: 05/20/2009
• Status: Abandoned Application

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Phillips, John, Hicks, Jennifer R., Tamulonis, John P., Martinell, Brian J., Williams, Edward J., Lowe, Brenda A.
• Application Number:
  US12/778,525
• Publication Number:
  US 20100299773A1
• Time in Patent Office: Days
• US Class Current: 800/260
• CPC Class Codes: A01H 1/045 using molecular markers
• CPC Class Codes: C12N 15/8201 Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
• CPC Class Codes: C12N 15/8205 Agrobacterium mediated transformation
• CPC Class Codes: C12N 15/8245 involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
• CPC Class Codes: C12N 15/8247 involving modified lipid metabolism, e.g. seed oil composition
• CPC Class Codes: C12N 15/8274 for herbicide resistance
• CPC Class Codes: C12N 15/8281 for bacterial resistance
• CPC Class Codes: C12N 15/8282 for fungal resistance
• CPC Class Codes: C12N 15/8283 for virus resistance
• CPC Class Codes: C12N 15/8286 for insect resistance
• CPC Class Codes: C12N 15/8289 Male sterility

Abstract

The invention is directed, in an embodiment, to a method for producing a transgenic plant comprising: providing a substantially homozygous plant line suitable for transformation; selecting a subline of the plant line having reduced heterogeneity; transforming plant materials from the subline with a transgenic construct that confers a desired trait to at least one transformed plant; recovering at least one transgenic event from the transformation step; and selecting a transgenic event exhibiting a desirable level of the desired trait using plants of the subline as control. The invention is also directed to a method for producing a plant having a desired trait comprising: providing a substantially homozygous plant line; selecting a subline of the plant line having reduced heterogeneity; crossing at least one individual of the selected subline with a donor parent having at least one desired trait to form at least one second individual having the desired trait; and backcrossing the at least one second individual with the at least one individual of the selected subline as a recurrent parent to form at least one progeny plant having the desired trait.

Claims

1. A method for producing a transgenic plant comprising:

a. providing a substantially homozygous plant line suitable for transformation;

b. selecting a subline of the plant line having reduced heterogeneity;

c. transforming plant materials from the subline with a transgenic construct that confers a desired trait to at least one transformed plant;

d. recovering at least one transgenic event from the transformation step; and

e. selecting a transgenic event exhibiting a desirable level of the desired trait using plants of the subline as control.

2. The method of claim 1 wherein the subline has an increased transformation efficiency as compared to the substantially homozygous plant line.

3. The method of claim 1 wherein the subline has a transformation efficiency which is at least about 20% greater than the substantially homozygous plant line.

4. The method of claim 1 wherein the subline has a transformation efficiency which is at least about 50% greater than the substantially homozygous plant line.

5. The method of claim 1 wherein the subline has a transformation efficiency which is at least about 75% greater than the substantially homozygous plant line.

6. The method of claim 1 wherein the at least one transformed plant has a reduced variability between transgenic events as compared to a plant that is transformed directly from the substantially homozygous plant line.

7. The method of claim 1 wherein the at least one transformed plant has more consistent expression levels of the desired trait as compared to a plant that is transformed directly from the substantially homozygous plant line.

8. The method of claim 1 wherein the substantially homozygous plant line is an inbred plant line.

9. The method of claim 1 wherein the subline is selected using marker-assisted selection.

10. The method of claim 1 wherein the transformed subline is further self-pollinated to form a progeny line.

11. The method of claim 10 wherein marker-assisted selection is performed on the substantially homozygous plant line and the progeny line to identify individuals from the progeny line that exhibit reduced heterogeneity when compared with the substantially homozygous plant line.

12. The method of claim 1 wherein the desired trait is selected from the group consisting of herbicide tolerance, increased yield, insect control, fungal disease resistance, virus resistance, nematode resistance, bacterial disease resistance, abiotic stress tolerance, quality grain, mycoplasma disease resistance, modified oils production, high oil production, high protein production, germination and seedling growth control, enhanced animal and human nutrition, low raffinose levels, environmental stress resistance, increased digestibility, increased industrial enzymes, increased pharmaceutical proteins, increased peptides and small molecules, improved processing traits, improved flavor, nitrogen fixation, hybrid seed production, and/or reduced allergenicity, biopolymers, and biofuels.

13. The method of claim 12 wherein the herbicide for which the herbicide tolerance is created is selected from the group consisting of glyphosate, dicamba, glufosinate, sulfonylurea, bromoxynil and norflurazon herbicides.

14. The method of claim 1 wherein the substantially homozygous plant line is selected from the group consisting of maize, cotton, peanut, barley, oats, orchard grass, rice, sorghum, sugar cane, tall fescue, turfgrass species, wheat, alfalfa, members of the genus Brassica, broccoli, cabbage, carrot, cauliflower, Chinese cabbage, cucumber, dry bean, eggplant, fennel, garden beans, gourd, leek, lettuce, melon, okra, onion, pea, pepper, pumpkin, radish, spinach, squash, sweet corn, tomato, watermelon, ornamental plants, and other fruit, vegetable, tuber, oilseed, and root crops, wherein oilseed crops include soybean, canola, oil seed rape, oil palm, sunflower, olive, corn, cottonseed, peanut, flaxseed, safflower, and coconut.

15. The method of claim 1, wherein the substantially homozygous plant line is selected from the group consisting of soybean, corn, cotton, canola, pepper, and tomato.

16. The method of claim 1, wherein the substantially homozygous plant line is selected from the group consisting of Glycine arenaria, Glycine argyrea, Glycine canescens, Glycine clandestine, Glycine curvata, Glycine cyrtoloba, Glycine falcate, Glycine latifolia, Glycine latrobeana, Glycine max, Glycine microphylla, Glycine pescadrensis, Glycine pindanica, Glycine rubiginosa, Glycine soja, Glycine sp., Glycine stenophita, Glycine tabacina, and Glycine tomentella.

17. A transgenic plant produced according to the method of claim 1.

18. The plant of claim 17, wherein the transformation efficiency of the subline is at least equivalent to that of the substantially homozygous plant line.

19. The plant of claim 17, wherein the transformation efficiency of the subline is greater than that of the substantially homozygous plant line.

20. A transgenic plant produced according to the method comprising:

a. providing a substantially homozygous plant line suitable for transformation;

b. selecting a subline of the plant line having reduced heterogeneity;

c. transforming plant materials from the subline with a transgenic construct that confers a desired trait to at least one transformed plant;

d. recovering at least one transgenic event from the transformation step; and

e. selecting a transgenic event exhibiting a desirable level of the desired trait using plants of the subline as control, wherein the transformation efficiency of the subline is at least about 20% greater than the transformation efficiency of the substantially homozygous plant line.

21. A method for producing a plant having a desired trait comprising:

a. providing a substantially homozygous plant line;

b. selecting a subline of the plant line having reduced heterogeneity;

c. crossing at least one individual of the selected subline with a donor parent having at least one desired trait to form at least one second individual having the desired trait; and

d. backcrossing the at least one second individual with the at least one individual of the selected subline as a recurrent parent to form at least one progeny plant having the desired trait.

22. The method of claim 21 wherein the backcrossing step is repeated until the at least one progeny plant has a genotype that is substantially identical to that of the selected subline.

23. The method of claim 21 further comprising the step of self-fertilizing the at least one progeny plant.

24. The method of claim 21 wherein the selected subline does not contain the desired trait prior to the crossing step.

25. A plant produced according to the method of claim 21.