METHODS AND COMPOSITIONS FOR SELECTING AN IMPROVED PLANT
First Claim
1. A method for producing a transgenic plant comprising:
- a. providing a substantially homozygous plant line suitable for transformation;
b. selecting a subline of the plant line having reduced heterogeneity;
c. transforming plant materials from the subline with a transgenic construct that confers a desired trait to at least one transformed plant;
d. recovering at least one transgenic event from the transformation step; and
e. selecting a transgenic event exhibiting a desirable level of the desired trait using plants of the subline as control.
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Abstract
The invention is directed, in an embodiment, to a method for producing a transgenic plant comprising: providing a substantially homozygous plant line suitable for transformation; selecting a subline of the plant line having reduced heterogeneity; transforming plant materials from the subline with a transgenic construct that confers a desired trait to at least one transformed plant; recovering at least one transgenic event from the transformation step; and selecting a transgenic event exhibiting a desirable level of the desired trait using plants of the subline as control. The invention is also directed to a method for producing a plant having a desired trait comprising: providing a substantially homozygous plant line; selecting a subline of the plant line having reduced heterogeneity; crossing at least one individual of the selected subline with a donor parent having at least one desired trait to form at least one second individual having the desired trait; and backcrossing the at least one second individual with the at least one individual of the selected subline as a recurrent parent to form at least one progeny plant having the desired trait.
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Citations
25 Claims
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1. A method for producing a transgenic plant comprising:
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a. providing a substantially homozygous plant line suitable for transformation; b. selecting a subline of the plant line having reduced heterogeneity; c. transforming plant materials from the subline with a transgenic construct that confers a desired trait to at least one transformed plant; d. recovering at least one transgenic event from the transformation step; and e. selecting a transgenic event exhibiting a desirable level of the desired trait using plants of the subline as control. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A transgenic plant produced according to the method comprising:
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a. providing a substantially homozygous plant line suitable for transformation; b. selecting a subline of the plant line having reduced heterogeneity; c. transforming plant materials from the subline with a transgenic construct that confers a desired trait to at least one transformed plant; d. recovering at least one transgenic event from the transformation step; and e. selecting a transgenic event exhibiting a desirable level of the desired trait using plants of the subline as control, wherein the transformation efficiency of the subline is at least about 20% greater than the transformation efficiency of the substantially homozygous plant line.
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21. A method for producing a plant having a desired trait comprising:
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a. providing a substantially homozygous plant line; b. selecting a subline of the plant line having reduced heterogeneity; c. crossing at least one individual of the selected subline with a donor parent having at least one desired trait to form at least one second individual having the desired trait; and d. backcrossing the at least one second individual with the at least one individual of the selected subline as a recurrent parent to form at least one progeny plant having the desired trait. - View Dependent Claims (22, 23, 24, 25)
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Specification