DATA COLLECTION SYSTEM AND METHOD FOR A FLOW CYTOMETER
First Claim
1. A system for a flow cytometer that collects data for a sample prepared with a plurality of fluorochromes comprising:
- a fixed gain detection system that collects data for a plurality of fluorescence channels, the detector having a wide dynamic range;
fluorochrome compensation factors for a plurality of fluorochromes types, wherein a fluorochrome compensation factor remains constant for the fixed gain detection system; and
a computer system that includes;
an interface that gathers fluorochrome information of the sample; and
an analysis program that compensates for spectral spillover in the collected data, using the fluorochrome compensation factors.
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Abstract
A system for a flow cytometer that collects data for a sample prepared with a plurality of fluorochromes that includes a fixed gain detection system that collects data for a plurality of fluorescence channels, fluorochrome compensation factors for a plurality of fluorochromes types, and a computer system that has an interface that gathers fluorochrome information of the sample and an analysis program that compensates for spectral spillover in the collected data. The fixed gain detection system preferably has a wide dynamic range. A fluorochrome compensation factor preferably remains constant for a fixed gain detection system. The analysis program preferably uses the fluorochrome compensation factors to compensate for spectral spillover.
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Citations
25 Claims
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1. A system for a flow cytometer that collects data for a sample prepared with a plurality of fluorochromes comprising:
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a fixed gain detection system that collects data for a plurality of fluorescence channels, the detector having a wide dynamic range; fluorochrome compensation factors for a plurality of fluorochromes types, wherein a fluorochrome compensation factor remains constant for the fixed gain detection system; and a computer system that includes; an interface that gathers fluorochrome information of the sample; and an analysis program that compensates for spectral spillover in the collected data, using the fluorochrome compensation factors. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A system for a flow cytometer comprising:
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a fixed gain detection system including; a detector that receives photonic inputs from an interrogation zone of the flow cytometer to sense a plurality of channels of fluorescence and that produces an analog signal, the detector having a wide dynamic range; and an analog-to-digital converter (ADC) coupled to the detector and that converts an analog signal to a digital signal, the ADC having a high bit resolution; and an analysis program that compensates for spectral spillover for sampled data, wherein the software uses stored fluorochrome compensation factors that are static for the detection system. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21)
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22. A method of collecting data in a flow cytometer system comprising:
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acquiring fluorochrome information for a sample through a computer interface; collecting sample data with a fixed gain detection system of the flow cytometer; accessing stored fluorochrome compensation factors associated with the acquired fluorochrome information; and compensating for spectral spillover using the accessed fluorochrome compensation factors. - View Dependent Claims (23, 24, 25)
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Specification