METHOD FOR CONTINUOUSLY DETECTING GLUCOSE CONCENTRATION IN SAMPLE, KIT THEREOF AND METHOD FOR USING BIOSENSOR
First Claim
1. A method for continuously detecting glucose concentration in a sample, comprising:
- (a) providing a biosensor comprising a transducer and a polysaccharide covered on the surface of the transducer;
(b) providing a carbohydrate binding protein solution, wherein the carbohydrate binding protein has at least one receptor, and the receptor is capable of binding to the polysaccharide and glucose;
(c) mixing a sample and the carbohydrate binding protein solution to form a mixture;
(d) contacting the mixture with the biosensor;
(e) detecting the amount of carbohydrate binding proteins bound to the polysaccharide by the biosensor, wherein glucose concentration of the sample is inversely proportional to the amount of carbohydrate binding proteins bound to the polysaccharide; and
(f) refreshing the surface of the biosensor with a high concentration glucose solution.
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Abstract
The invention provides a method for continuously detecting glucose concentration in a sample, including: (a) providing a biosensor comprising a transducer and a polysaccharide covered on the surface of the transducer; (b) providing a carbohydrate binding protein solution, wherein the carbohydrate binding protein has at least one receptor, and the receptor is capable of binding to the polysaccharide and glucose; (c) mixing a sample and the carbohydrate binding protein solution to form a mixture; (d) contacting the mixture with the biosensor; (e) detecting the amount of carbohydrate binding proteins bound to the polysaccharide by the biosensor, wherein glucose concentration of the sample is inversely proportional to the amount of carbohydrate binding proteins bound to the polysaccharide; and (f) refreshing the surface of the biosensor with a high concentration glucose solution.
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Citations
32 Claims
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1. A method for continuously detecting glucose concentration in a sample, comprising:
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(a) providing a biosensor comprising a transducer and a polysaccharide covered on the surface of the transducer; (b) providing a carbohydrate binding protein solution, wherein the carbohydrate binding protein has at least one receptor, and the receptor is capable of binding to the polysaccharide and glucose; (c) mixing a sample and the carbohydrate binding protein solution to form a mixture; (d) contacting the mixture with the biosensor; (e) detecting the amount of carbohydrate binding proteins bound to the polysaccharide by the biosensor, wherein glucose concentration of the sample is inversely proportional to the amount of carbohydrate binding proteins bound to the polysaccharide; and (f) refreshing the surface of the biosensor with a high concentration glucose solution. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A kit for continuously detecting glucose concentration in a sample, comprising:
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a biosensor comprising a transducer and a polysaccharide covered on the surface of the transducer; a reactive solution for reacting with the sample, wherein the reactive solution comprises a carbohydrate binding protein solution with a particular concentration; and a washing solution for refreshing the surface of the biosensor, wherein the washing solution comprises a high concentration glucose solution. - View Dependent Claims (15, 16, 17, 18, 19, 20)
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21. A method for using a biosensor, comprising:
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(a) providing a biosensor comprising a transducer and a polysaccharide covered on the surface of the transducer; (b) contacting a mixture of a reactive solution comprising a carbohydrate binding protein solution with a particular concentration and a sample with the biosensor, wherein the carbohydrate is capable of binding to the polysaccharide and glucose; (c) detecting the amount of carbohydrate binding proteins bound to the polysaccharide by the biosensor to obtain glucose concentration of the sample; and (d) refreshing the surface of the biosensor with a washing solution, wherein the washing solution comprises a high concentration glucose solution. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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Specification