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METHODS, COMPOSITIONS AND KITS FOR THE IMPROVED DETECTION OF SMALL RNA MOLECULES

  • US 20100304442A1
  • Filed: 10/08/2008
  • Published: 12/02/2010
  • Est. Priority Date: 10/09/2007
  • Status: Active Grant
First Claim
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1. A method for reverse transcription of a small RNA target sequence less than about 100 nucleotides in length, which comprises:

  • (a) contacting a sample comprising the small RNA target sequence with;

    (i) a primer that is sufficiently complementary the small RNA target sequence to hybridize thereto, and(ii) an RNA-dependent DNA polymerase having reverse transcriptase activity; and

    (b) synthesizing cDNA complementary to the small RNA target sequence in the presence of a mixture of dNTPs comprising at least one base-modified, duplex-stabilizing dNTP that is a substrate for the RNA-dependent DNA polymerase.

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