CELL ROLLING SEPARATION
First Claim
Patent Images
1. A method comprising steps of:
- providing a surface that is at least partially coated with an ordered layer of cell adhesion molecules, wherein the surface comprises at least one edge between an area coated with the ordered layer and another area that is not coated with the ordered layer; and
flowing a population of cells across the surface in a direction which forms a non-zero angle α
s with the at least one edge, wherein at least one cell in the population of cells comprises a surface moiety that is recognized by the cell adhesion molecules and wherein the at least one cell rolls for a period of time in a direction that is α
s to the direction of flow as a result of interacting with at least a portion of the at least one edge.
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Abstract
The present invention provides systems for cell separation based on cell rolling on surfaces along edges of regions coated with cell adhesion molecules. A variety of designs of coated regions and edges are disclosed.
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Citations
157 Claims
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1. A method comprising steps of:
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providing a surface that is at least partially coated with an ordered layer of cell adhesion molecules, wherein the surface comprises at least one edge between an area coated with the ordered layer and another area that is not coated with the ordered layer; and flowing a population of cells across the surface in a direction which forms a non-zero angle α
s with the at least one edge, wherein at least one cell in the population of cells comprises a surface moiety that is recognized by the cell adhesion molecules and wherein the at least one cell rolls for a period of time in a direction that is α
s to the direction of flow as a result of interacting with at least a portion of the at least one edge. - View Dependent Claims (2, 3, 4, 9, 16, 19, 20, 21, 22, 23, 24, 33, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 64, 67, 70, 74, 82, 100, 104, 106, 111, 113, 116)
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5-8. -8. (canceled)
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10-15. -15. (canceled)
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17-18. -18. (canceled)
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25-29. -29. (canceled)
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31-32. -32. (canceled)
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34-48. -48. (canceled)
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60-63. -63. (canceled)
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65-66. -66. (canceled)
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68-69. -69. (canceled)
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71-73. -73. (canceled)
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75-81. -81. (canceled)
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83-99. -99. (canceled)
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101-103. -103. (canceled)
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105. (canceled)
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107-110. -110. (canceled)
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112. (canceled)
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114-115. -115. (canceled)
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117-123. -123. (canceled)
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124. A device comprising:
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a separation flow chamber, wherein the separation flow chamber comprises a surface that is at least partially coated with an ordered layer of cell adhesion molecules, and the surface comprises at least one edge between an area coated with the ordered layer and another area that is not coated with the ordered layer; an inlet for flowing cells into the separation flow chamber; and an outlet for flowing cells out of the separation flow chamber; wherein when cells are flowed through the inlet to the outlet they flow at an angle α
s to the direction of the at least one edge unless they interact with the at least one edge.
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125-155. -155. (canceled)
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156. A method comprising steps of:
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providing a three dimensional surface that is at least partially coated with an ordered layer of cell adhesion molecules, and flowing a population of cells across the surface in such conditions to create a stagnation line of no flow, wherein the direction of flow forms a non-zero angle α
s with the stagnation line, wherein at least one cell in the population of cells comprises a surface moiety that is recognized by the cell adhesion molecules and wherein at least one cell in the population of cells rolls at least part of the time in a direction that is α
s to the direction of flow.
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157-158. -158. (canceled)
Specification