MICROCHIP REACTION DETECTION SYSTEM AND METHOD OF REACTION OF MICROCHIP IN FLOW PATH

• US 20100304498A1
• Filed: 09/22/2007
• Published: 12/02/2010
• Est. Priority Date: 10/18/2006
• Status: Abandoned Application

• Current Assignee: Konica Minolta Medical & Graphic Incorporated (Konica Minolta Inc.)
• Original Assignee: Konica Minolta Medical & Graphic Incorporated (Konica Minolta Inc.)
• Inventors: Aoki, Youichi, Higashino, Kusunoki, Sando, Yasuhiro, Nakajima, Akihisa
• Application Number:
  US12/445,709
• Publication Number:
  US 20100304498A1
• Time in Patent Office: Days
• US Class Current: 436/501
• CPC Class Codes: B01J 2219/00286 Reactor vessels with top and bottom openings
• CPC Class Codes: B01J 2219/00353 Pumps
• CPC Class Codes: B01J 2219/00495 Means for heating or cooling the reaction vessels
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00702 Processes involving means for analysing and characterising the products
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/0074 Biological products
• CPC Class Codes: B01L 2200/0673 Handling of plugs of fluid surrounded by immiscible fluid
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/502784 specially adapted for droplet or plug flow, e.g. digital microfluidics automatic analysis using a stream of discrete samples in a tube system G01N35/08
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: G01N 2035/00158 Elements containing microarrays, i.e. "biochip"
• CPC Class Codes: G01N 2035/00366 Several different temperatures used
• CPC Class Codes: G01N 2035/0097 monitoring reactions as a function of time
• CPC Class Codes: G01N 35/08 using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis

Abstract

Disclosed is a microchip reaction detection system comprising: a microchip comprising a driving liquid injection unit into which a driving liquid for driving a solution is injected and a detection unit on which a substance to be reacted is supported and where the reaction between the substance and the solution is detected; a driving liquid pump for injecting the driving liquid into the driving liquid injection unit; an air pump for injecting a gas into the detection unit; and a control unit for controlling the driving liquid pump so that the driving liquid can be injected into the driving liquid injection unit and also controlling the air pump so that the gas can be injected into the detection unit intermittently in an amount at least effective for pushing away the solution injected in the detection unit while feeding the solution to the detection unit.

Claims

1. A microchip reaction detection system comprising:

a microchip comprising

a driving liquid injection unit into which a driving liquid for driving a solution is injected and

a detection unit on which a reacting substance is supported and in which the reaction between the reacting substance and the solution is detected;

a driving liquid pump for injecting the driving liquid into the driving liquid injection unit;

an air pump for injecting a gas into the detection unit; and

a control unit

for controlling the driving liquid pump so that the driving liquid can be injected into the driving liquid injection unit and controlling the air pump so that the gas can be injected into the detection unit intermittently of at least an amount effective for pushing away the solution injected into the detection unit while feeding the solution to the detection unit.

2. The microchip reaction detection system of claim 1,

wherein the microchip has a flow path for injecting the gas which flow path joins a flow path for feeding the solution at the detection unit.

3. A method of reaction inside a flow path of a microchip,

wherein the reaction is carried out

by feeding a solution to a wall of a flow path which supports a reacting substance in the detection unit and

by contacting the reacting substance with the solution, and

wherein the solution and a gas of at least an amount effective for pushing out the solution injected into the detection unit are alternatively injected into the detection unit at the time of the reaction.

4. The method of reaction inside the flow path of the microchip of claim 3,

wherein the reaction between the reacting substance and the solution is an avidin-biotin reaction.

5. The method of reaction inside the flow path of the microchip of claim 3,

wherein the reaction between the reacting substance and the solution is a hybridization reaction.

6. The method of reaction inside the flow path of the microchip of claim 3,

wherein the reaction between the reacting substance and the solution is an antigen antibody reaction.