Polypeptides having Cellulolytic Enhancing Activity and Polynucleotides Encoding Same
First Claim
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1. An isolated polypeptide having cellulolytic enhancing activity, selected from the group consisting of:
- (a) a polypeptide comprising an amino acid sequence having at least 60% identity to the mature polypeptide of SEQ ID NO;
2;
(b) a polypeptide encoded by a polynucleotide that hybridizes under at least medium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO;
1, (ii) the cDNA sequence contained in the mature polypeptide coding sequence of SEQ ID NO;
1, or (iii) a full-length complementary strand of (i) or (ii);
(c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 60% identity to the mature polypeptide coding sequence of SEQ ID NO;
1; and
(d) a variant comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO;
2.
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Abstract
The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
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Citations
20 Claims
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1. An isolated polypeptide having cellulolytic enhancing activity, selected from the group consisting of:
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(a) a polypeptide comprising an amino acid sequence having at least 60% identity to the mature polypeptide of SEQ ID NO;
2;(b) a polypeptide encoded by a polynucleotide that hybridizes under at least medium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO;
1, (ii) the cDNA sequence contained in the mature polypeptide coding sequence of SEQ ID NO;
1, or (iii) a full-length complementary strand of (i) or (ii);(c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 60% identity to the mature polypeptide coding sequence of SEQ ID NO;
1; and(d) a variant comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO;
2. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 17, 18, 19, 20)
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14. A nucleic acid construct comprising a gene encoding a protein operably linked to a nucleotide sequence encoding a signal peptide comprising or consisting of amino acids 1 to 18 of SEQ ID NO:
- 2, wherein the gene is foreign to the nucleotide sequence.
- View Dependent Claims (15, 16)
Specification