NON-TARGET AMPLIFICATION METHOD FOR DETECTION OF RNA SPLICE-FORMS IN A SAMPLE
First Claim
Patent Images
1. A method of detecting the presence of a target RNA, the method comprising:
- a) providing at least one DNA capture probe, wherein the at least one DNA capture probe is bound to a support;
b) hybridizing the target RNA to said at least one DNA capture probe, yielding a target RNA;
DNA capture probe complex;
c) isolating the target RNA;
DNA capture probe complex;
d) providing at least one DNA amplification probe, and hybridizing said at least one DNA amplification probe to said target RNA;
DNA capture probe complex, yielding a target RNA;
DNA capture/amplification probe complex;
e) providing an anti-RNA;
DNA hybrid antibody, and incubating said target RNA;
DNA capture/amplification probe complex with said antibody, yielding a target RNA;
DNA;
antibody complex;
f) detecting said antibody, wherein said detecting indicates the presence of said target RNA.
1 Assignment
0 Petitions
Accused Products
Abstract
Provided are methods of isolating RNA from a biological sample, methods and means for determining the presence of particular RNA splice-form variants in a biological sample, methods and means for determining the relative ratio of RNA ratios in a biological sample, and methods and means for predicting the progression of precancerous cervical lesions.
-
Citations
26 Claims
-
1. A method of detecting the presence of a target RNA, the method comprising:
-
a) providing at least one DNA capture probe, wherein the at least one DNA capture probe is bound to a support; b) hybridizing the target RNA to said at least one DNA capture probe, yielding a target RNA;
DNA capture probe complex;c) isolating the target RNA;
DNA capture probe complex;d) providing at least one DNA amplification probe, and hybridizing said at least one DNA amplification probe to said target RNA;
DNA capture probe complex, yielding a target RNA;
DNA capture/amplification probe complex;e) providing an anti-RNA;
DNA hybrid antibody, and incubating said target RNA;
DNA capture/amplification probe complex with said antibody, yielding a target RNA;
DNA;
antibody complex;f) detecting said antibody, wherein said detecting indicates the presence of said target RNA. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
-
-
11. The method of claim 11, wherein the at least one DNA capture probe and the at least one DNA amplification probe are complementary to RNA from HPV high risk types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68, 26, 66, 73, and 82.
-
12. A kit for the detection of a target RNA, the kit comprising:
-
a) at least One DNA capture probe, bound to a magnetic support; b) at least one DNA amplification probe; c) an anti-RNA;
DNA hybrid antibody; andd) a detection reagent. - View Dependent Claims (13, 14)
-
-
15. A method of providing target RNA for detection, the method comprising:
-
a) incubating a biological sample containing the target RNA with carboxyl beads; b) isolating the beads; c) lysing the biological sample attached to the isolated beads; and d) isolating the beads from the lysed biological sample, wherein the resulting supernatant contains the target RNA for detection.
-
-
16. A method of detecting the presence of a target RNA, the method comprising:
-
a) providing at least one DNA capture probe; b) providing a first anti-RNA;
DNA hybrid antibody, wherein the first anti-RNA;
DNA hybrid antibody is bound to a support;c) hybridizing the target RNA to said at least one DNA capture probe, yielding a target RNA;
DNA capture probe complex;d) incubating said target RNA;
DNA capture probe complex with said anti-RNA;
DNA hybrid antibody, yielding a bound target RNA;
DNA capture probe complex;e) providing at least one DNA amplification probe, and hybridizing said at least one DNA amplification probe to said bound target RNA;
DNA capture probe complex, yielding a bound target RNA;
DNA capture/amplification probe complex;f) providing a second anti-RNA;
DNA hybrid antibody, and incubating said bound target RNA;
DNA capture/amplification probe complex with said second anti-RNA;
DNA hybrid antibody, yielding a bound target RNA;
DNA;
antibody complex;g) detecting said second anti-RNA;
DNA hybrid antibody, wherein said detecting indicates the presence of said target RNA. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
-
Specification