TEST STRIP AND METHOD FOR MEASURING BLOOD CHOLESTEROL LEVEL USING THE SAME

• US 20100311091A1
• Filed: 03/11/2010
• Published: 12/09/2010
• Est. Priority Date: 06/04/2009
• Status: Active Grant

• Current Assignee: Osang Healthcare Co., Ltd.
• Original Assignee: INFOPIA CO., LTD
• Inventors: RYU, Ji-Eon, KHO, Byung-Hoon, LEE, Sung-Dong, JOUNG, Hyou-Arm, BAE, Byeong-Woo, KIM, Jin-Kyeong
• Granted Patent: US 8,377,684 B2
• Time in Patent Office: Days
• US Class Current: 435/11
• CPC Class Codes: C12Q 1/60 involving cholesterol
• CPC Class Codes: G01N 33/526 the element being adapted for a specific analyte
• CPC Class Codes: G01N 33/92 involving lipids, e.g. cholesterol , lipoproteins, or their receptors steroid hormones G01N33/743

Abstract

Disclosed are a test strip and a method for measuring medical data. The test strip comprises an upper cover having one or more application holes; a lower support having one or more detected parts at positions corresponding to detecting units of the measuring apparatus; an erythrocyte and LDL cholesterol-filtering layer designed to filter off both of erythrocytes and LDL cholesterol from an applied blood sample by agglutinating erythrocytes and precipitating LDL cholesterol; and a reaction layer in which the blood sample free of erythrocytes and LDL cholesterol is reacted with an reagent, the filtering layer and the reaction layer being stacked between the lower support and the upper cover. Structured to filter off erythrocytes and low-density lipoprotein (LDL) cholesterol from a blood sample in a single layer, the test strip of the present invention can be made to be slim and allows a low volume of blood to be analyzed.

Claims

1. A test strip for measuring medical data in association with a measuring apparatus, comprising:

an upper cover having one or more application holes;

a lower support having one or more detected parts at positions corresponding to detecting units of the measuring apparatus;

an erythrocyte and low-density lipoprotein (LDL) cholesterol-filtering layer designed to filter off both of erythrocytes and low-density lipoprotein (LDL) cholesterol from an applied blood sample by agglutinating erythrocytes and precipitating LDL cholesterol; and

a reaction layer in which the blood sample free of erythrocytes and low-density lipoprotein (LDL) cholesterol is reacted with an reagent, the filtering layer and the reaction layer being stacked between the lower support and the upper cover.

2. The test strip according to claim 1, wherein the erythrocyte and low-density lipoprotein (LDL) cholesterol-filtering layer contains a hematogglutinin selected from a group consisting of lectin, a cationic polymer, a saccharide and a combination thereof.

3. The test strip according to claim 1, wherein the erythrocyte and low-density lipoprotein (LDL) cholesterol-filtering layer contains phosphotungstic acid (PTA) as an LDL cholesterol-precipitating agent.

4. The test strip according to claim 1, wherein the erythrocyte and low-density lipoprotein (LDL) cholesterol-filtering layer contains dextran sulfate as a low-density lipoprotein (LDL) cholesterol-precipitating agent.

5. The test strip according to claim 1, wherein the erythrocyte and low-density lipoprotein (LDL) cholesterol-filtering layer contains as a low-density lipoprotein (LDL) cholesterol-precipitating agent, a combination of a divalent cation and one selected from a group consisting of phosphotungstic acid (PTA), dextran sulfate and a combination thereof.

6. A method for detecting cholesterol, comprising:

filtering off erythrocytes and low-density lipoprotein (LDL) cholesterol from a blood sample; and

reacting the blood sample free of erythrocytes and low-density lipoprotein (LDL) cholesterol with a reagent to generate medical data.

7. The method according to claim 6, wherein the filtering step is carried out by using a hemagglutinating agent selected from a group consisting of lectin, a cationic polymer, a saccharide and a combination thereof.

8. The method according to claim 6, wherein the filtering step is carried out by using phosphotungtic acid (PTA) as a low-density lipoprotein (LDL) cholesterol-precipitating agent.

9. The method according to claim 6, wherein the filtering step is carried out by using dextran sulfate as a low-density lipoprotein (LDL) cholesterol-precipitating agent.

10. The method according to claim 6, wherein the filtering step is carried out by using as a low-density lipoprotein (LDL) cholesterol-precipitating agent, a combination of a divalent cation and one selected from a group consisting of phosphotungstic acid (PTA), dextran sulfate and a combination thereof.