METHOD AND DEVICE FOR FILTERING PLATELETS

• US 20100316988A1
• Filed: 06/02/2010
• Published: 12/16/2010
• Est. Priority Date: 06/15/2009
• Status: Active Grant

• Current Assignee: Biovec Transfusion LLC
• Original Assignee: Biovec Transfusion LLC
• Inventors: SEHGAL, Lakshman R.
• Granted Patent: US 9,095,818 B2
• Time in Patent Office: Days
• US Class Current: 435/2
• CPC Class Codes: B01D 2313/66 Biodegradability of parts of the module
• CPC Class Codes: B01D 2315/16 Diafiltration
• CPC Class Codes: B01D 2323/39 Electrospinning
• CPC Class Codes: B01D 2325/04 Characteristic thickness
• CPC Class Codes: B01D 2325/20 Specific permeability or cut-off range
• CPC Class Codes: B01D 61/145 Ultrafiltration
• CPC Class Codes: B01D 61/243 Dialysis
• CPC Class Codes: B01D 61/246 Membrane extraction
• CPC Class Codes: B01D 63/02 Hollow fibre modules
• CPC Class Codes: B01D 69/02 characterised by their properties
• CPC Class Codes: B01D 69/08 Hollow fibre membranes manufacture of hollow fibres D01D5/24, D01F1/08

Abstract

The present invention relates to methods for removing antiplatelet agents and anticoagulants from a platelet preparation. In one embodiment, the method includes the step of flowing the platelet preparation through a filtering tube comprising a filtering membrane and separating the antiplatelet agents and anticoagulants from the platelet preparation by tangential flow filtration. In another embodiment, the method includes the step of passing the platelet preparation through porous material that specifically binds to the antiplatelet agents and anticoagulants.

Claims

1. A method for removing antiplatelet agents and anticoagulants from a platelet preparation, comprising:

flowing the platelet preparation through a filtering tube comprising a filtering membrane and separating the antiplatelet agents and anticoagulants from the platelet preparation by tangential flow filtration.

2. The method of claim 1, wherein the filter membrane comprises a material selected from the group consisting of regenerated cellulose, cellulose acetate, polyamide, polysulfone, polyethylsulfone and combinations thereof.

3. The method of claim 2, wherein the filter membrane comprises polysulfone or polyethylsulfone.

4. The method of claim 1, wherein the filter membrane has a pore size ranging from molecular cut off of 3000 daltons to 0.5 micron.

5. The method of claim 1, wherein the filtering tube has an inner diameter of at least 0.5 mm.

6. The method of claim 1, wherein the platelet preparation flows through the filtering tube at a flow rate of 150 to 400 ml/min.

7. The method of claim 1, wherein an extraction liquid is circulated outside the filtering tube in a counter current manner.

8. The method of claim 7, wherein the extraction fluid comprises 0.9% w/v sodium chloride.

9. A method for removing antiplatelet agents and anticoagulants from a platelet preparation, comprising:

passing the platelet preparation through porous material that specifically binds to the antiplatelet agents and anticoagulants.

10. The method of claim 9, wherein the porous material comprises a nanofiber.

11. The method of claim 10, wherein the nanofiber is a cellulose nanofiber.

12. The method of claim 11, wherein the cellulose nanofiber has a diameter between 5-60 nm.

13. The method of claim 10, wherein the cellulose nanofiber is modified to provide specific binding sites for a given antiplatelet agent or an anticoagulant.

14. The method of claim 9, wherein the porous material comprises a reinforced composite film comprising 90% polyvinyl alcohol and 10% nanofiber.

15. The method of claim 9, wherein the nanofiber is a biodegradable nanofiber.

16. The method of claim 15, wherein the biodegradable nanofiber comprises poly(glycolic acid) (PGA), poly(L-lactic acid) (PLLA), poly(lactic-co-glycolic acid) (PLGA), or combinations thereof.

17. The method of claim 15, wherein the surface of the biodegradable nanofiber is chemically modified by oxygen plasma treatment and in situ grafting of hydrophilic acrylic acid (AA).

18. The method of claim 15, wherein the biodegradable nanofiber forms a porous filter with a thickness of 200-800 nm, a pore size of 2-30 micron, and a porosity of 94-96%.

19. The method of claim 10, wherein the nanofiber is a carbon nanofiber.

20. The method of claim 19, wherein the carbon nanofiber is a chemically modified carbon nanofiber.

21. A method for antiplatelet agents and anticoagulants from a platelet preparation using diafiltration, comprising:

circulating the platelet preparation through a hollow fiber membrane capable of separating the antiplatelet agents and anticoagulants from the platelet preparation,

wherein a diafiltration buffer is added to the platelet preparation during circulation to maintain a constant volume of the platelet preparation.

22. The method of claim 21, wherein the hollow fiber membrane comprises a material selected from the group consisting of regenerated cellulose, cellulose acetate, polyamide, polyurethane, polypropylene, polysulfone, polyethersulfone, polycarbonate, nylon, polyimide and combinations thereof.

23. The method of claim 21, wherein the hollow fiber membrane comprises polysulfone or polyethylsulfone.

24. The method of claim 21, wherein the hollow filter membrane has an inner diameter of at least 0.5 mm and a pore size ranging from molecular cut off of 3000 daltons to 0.5 micron.

25. A method for removing antiplatelet agents and anticoagulants from a platelet preparation, comprising:

flowing the platelet preparation through the surface of a filtering membrane and separating the antiplatelet agents and anticoagulants from the platelet preparation by tangential flow filtration.

26. The method of claim 25, wherein the filtering membrane has a pore size ranging from molecular cut off of 3000 daltons to 0.5 micron.

27. A filter for removing antiplatelet agents and anticiagulants from a platelet preparation, comprising:

a nanofiber modified to bind specifically to an antiplatelet agent or an anticoagulant.

28. The filter of claim 27, wherein the nanofiber is selected from the group consisting of cellulose nanofibers, biodegradable nanofibers and carbon nanofibers.