Methods For Detecting Biomarkers
First Claim
1. A method of detecting biomarkers, comprising a) providing a mixture comprising first and second beads, said first bead comprising a first mass tag and a first coupling agent, said second bead comprising a second mass tag and a second coupling agent, wherein said first and second coupling agents and said first and second mass tags are different;
- b) contacting said first and second coupling agents with a solution comprising a biomarker;
c) making an array with said beads; and
d) subjecting said array to mass spec analysis under conditions wherein said biomarker is detected.
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Abstract
Methods of amplifying nucleic acid on a solid support are described. Beads and template, each in known concentrations, are employed so a range of template to bead ratios can be exploited. Where the beads contain primers, the template can be amplified. After amplification, non-covalently bound template is removed, so as to leave beads with extended primers (or beads with primers that were not extended).
47 Citations
46 Claims
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1. A method of detecting biomarkers, comprising a) providing a mixture comprising first and second beads, said first bead comprising a first mass tag and a first coupling agent, said second bead comprising a second mass tag and a second coupling agent, wherein said first and second coupling agents and said first and second mass tags are different;
- b) contacting said first and second coupling agents with a solution comprising a biomarker;
c) making an array with said beads; and
d) subjecting said array to mass spec analysis under conditions wherein said biomarker is detected. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
- b) contacting said first and second coupling agents with a solution comprising a biomarker;
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10. A method of detecting biomarkers, comprising a) providing a mixture comprising first and second beads, said first bead comprising a first mass tag and a first coupling agent, said second bead comprising a second mass tag and a second coupling agent, wherein said first and second coupling agents and said first and second mass tags are different;
- b) making an array with said beads;
c) contacting said first and second coupling agents with a solution comprising a biomarker; and
d) subjecting said array to mass spec analysis under conditions wherein said biomarker is detected. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18)
- b) making an array with said beads;
- 19. An array comprising first and second beads, said first bead comprising a first mass tag and a first coupling agent, said second bead comprising a second mass tag and a second coupling agent, wherein said first and second coupling agents are different and said first and second mass tags are of different mass, said array further comprising a biomarker bound to said first coupling agent.
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24. A method of detecting ligand binding, comprising a) providing a mixture comprising first and second beads, said first bead comprising a first mass tag and a first biomolecule, said second bead comprising a second mass tag and a second biomolecule, wherein said first and second biomolecules and said first and second mass tags are different;
- b) contacting said first and second biomolecules with a solution comprising a ligand;
c) making an array with said beads; and
d) subjecting said array to mass spec analysis under conditions wherein binding of said ligand to a biomolecule is detected. - View Dependent Claims (25, 26, 27, 28, 29, 30, 31)
- b) contacting said first and second biomolecules with a solution comprising a ligand;
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32. A method of detecting ligand binding, comprising a) providing a mixture comprising first and second beads, said first bead comprising a first mass tag and a first biomolecule, said second bead comprising a second mass tag and a second biomolecule, wherein said first and second biomolecules and said first and second mass tags are different;
- b) making an array with said beads;
c) contacting said first and second biomolecules with a solution comprising a ligand; and
d) subjecting said array to mass spec analysis under conditions wherein binding of said ligand to a biomolecule is detected. - View Dependent Claims (33, 34, 35, 36, 37, 38, 39, 40, 41)
- b) making an array with said beads;
- 42. An array comprising first and second beads, said first bead comprising a first mass tag and a first biomolecule, said second bead comprising a second mass tag and a second biomolecule, wherein said first and second biomolecules are different and said first and second mass tags are of different mass, said array further comprising a ligand bound to said first biomolecule.
Specification