DETECTION AND FLUIDIC SYSTEM OF A FLOW CYTOMETER
First Claim
1. A fluidic system for pumping sample container into an interrogation zone of a flow cytometer, comprising:
- a sheath pump that pumps sheath fluid from a sheath container into the interrogation zone of the flow cytometer;
a waste pump that pumps waste fluid from the interrogation zone into a waste container;
wherein the flow rate of the sheath fluid is different from the flow rate of the waste fluid, thereby drawing the sample fluid from the sample container into the interrogation zone;
a detection system coupled to the interrogation zone that provides a data set of input signals from the sample fluid;
an analysis engine that recognizes aggregate particle events in the data set; and
a controller that automatically adjusts the flow rate of the sample fluid into the interrogation zone based on the recognition of aggregate particle events by controlling at least one of the flow rates of the sheath fluid and the waste fluid.
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Accused Products
Abstract
The fluidic system including a sheath pump that pumps sheath fluid from a sheath container into an interrogation zone, a waste pump that pumps waste fluid from the interrogation zone to a waste container, in which the flow rate of the sheath fluid is different from the flow rate of the waste fluid thereby drawing a sample fluid from a sample container into the interrogation zone, a detection system that provides a data set of input signals from the sample fluid, an analysis engine that recognizes aggregate particle events in the data set, and a controller that automatically adjusts the flow rate of the sample fluid into the interrogation zone based on the recognition of aggregate particle events, by controlling at least one of the flow rates of the sheath fluid and the waste fluid.
124 Citations
21 Claims
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1. A fluidic system for pumping sample container into an interrogation zone of a flow cytometer, comprising:
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a sheath pump that pumps sheath fluid from a sheath container into the interrogation zone of the flow cytometer; a waste pump that pumps waste fluid from the interrogation zone into a waste container; wherein the flow rate of the sheath fluid is different from the flow rate of the waste fluid, thereby drawing the sample fluid from the sample container into the interrogation zone; a detection system coupled to the interrogation zone that provides a data set of input signals from the sample fluid; an analysis engine that recognizes aggregate particle events in the data set; and a controller that automatically adjusts the flow rate of the sample fluid into the interrogation zone based on the recognition of aggregate particle events by controlling at least one of the flow rates of the sheath fluid and the waste fluid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for pumping sample fluid from a sample container into an interrogation zone of a flow cytometer, comprising:
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simultaneously pumping sheath fluid from the sheath container into the interrogation zone of the flow cytometer and pumping waste fluid from the interrogation zone into a waste container, wherein the flow rate of the sheath fluid is different from the flow rate of the waste fluid, thereby drawing the sample fluid from the sample container into the interrogation zone; collecting a data set of input signals from the sample fluid; recognizing aggregate particle events in the data set with use of an algorithm; and automatically adjusting the flow rate of the sample fluid into the interrogation zone based on the recognition of aggregate particle events in the data set, wherein adjusting the flow rate of the sample fluid includes controlling at least one of the flow rates of the sheath fluid and the waste fluid. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A fluidic system for pumping a sample fluid from a sample container into an interrogation zone of a flow cytometer, comprising:
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a pump system that pumps a sample fluid from a sample container into the interrogation zone; a detection system coupled to the interrogation zone that provides a data set of input signals from the sample fluid; an analysis engine that recognizes aggregate particle events in the data set; and a controller that automatically adjusts the flow rate of the sample fluid into the interrogation zone based on the recognition of aggregate particle events.
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Specification