CULTURING AND DIFFERENTIATING NEURAL PRECURSOR CELLS
First Claim
1. A method for culturing neural precursor cells comprising the steps of:
- (a) isolating tissue comprising neural precursor cells from an animal subject;
(b) dissociating the tissue to single cells; and
(c) attaching the single cells to a substrate in medium comprising pituitary extract and mitogenic factors EGF and bFGF, for a duration of time sufficient to produce a culture comprising at least one cell type that is proliferating and/or differentiated.
0 Assignments
0 Petitions
Accused Products
Abstract
Systems and methods have been developed for large-scale propagation and differentiation of populations of neurons and glia from neural precursor cells derived from postnatal brain. Under culture conditions containing pituitary extract and mitogenic factors, cells derived from neural stem cells can be attached to a substrate, maintained and serially passaged in culture. Upon removal of mitogenic factors, clusters of neural progenitor cells can be induced that co-express markers of neural stem cells and immature neurons. Unlimited numbers of cells at characterized stages of neurogenesis can be produced. Upon maturation, neuronal cells extend processes and differentiate into mature neuronal phenotypes capable of generating action potentials.
-
Citations
18 Claims
-
1. A method for culturing neural precursor cells comprising the steps of:
-
(a) isolating tissue comprising neural precursor cells from an animal subject; (b) dissociating the tissue to single cells; and (c) attaching the single cells to a substrate in medium comprising pituitary extract and mitogenic factors EGF and bFGF, for a duration of time sufficient to produce a culture comprising at least one cell type that is proliferating and/or differentiated. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
-
-
18-27. -27. (canceled)
Specification