Methods of Using Dyes in Association with Nucleic Acid Staining or Detection and Associated Technology
First Claim
1. A method of staining a sample that is exposed to a matrix or a surface, wherein if nucleic acid is present in the sample it is capable of being immobilized relative to the matrix or the surface, comprising:
- providing an aqueous solution comprising a fluorescent nucleic acid dye and a salt that comprises an anion that would be sufficient as a component of a strong acid and a cation that would be sufficient as a component of a strong base; and
exposing the sample to the aqueous solution.
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Abstract
Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. Such a dye and a salt that comprises an anion that is associated with a strong acid and a cation that is associated with a strong base may be used in such an application. A dimeric dye, such as a dimeric dye capable of forming a hairpin-like structure, may be used to stain and/or detect nucleic acids via a release-on-demand mechanism. A dimeric dye having low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, may be used to stain and/or detect nucleic acids.
44 Citations
6 Claims
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1. A method of staining a sample that is exposed to a matrix or a surface, wherein if nucleic acid is present in the sample it is capable of being immobilized relative to the matrix or the surface, comprising:
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providing an aqueous solution comprising a fluorescent nucleic acid dye and a salt that comprises an anion that would be sufficient as a component of a strong acid and a cation that would be sufficient as a component of a strong base; and exposing the sample to the aqueous solution. - View Dependent Claims (2, 3, 4, 5, 6)
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Specification